6V62

SETD3 double mutant (N255F/W273A) in Complex with an Actin Peptide with His73 Replaced with Lysine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	Y	Actin, cytoplasmic 1	polymer	23	2859.3	1	UniProt (C9JUM1) UniProt (by SIFTS) (P63261) In PDB	Homo sapiens
2	A	Actin-histidine N-methyltransferase	polymer	596	67404.1	1	UniProt (Q86TU7) Pfam (PF00856) Pfam (PF09273) In PDB	Homo sapiens (Human)	SET domain-containing protein 3,hSETD3
3	A	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
5		water	water		18.0	187	Chemie (HOH)

Sequence modifications

Y: 66 - 88 (UniProt: C9JUM1)

PDB	External Database	Details
Lys 73	His 73	engineered mutation

A: 0 - 593 (UniProt: Q86TU7)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
Phe 255	Asn 256	engineered mutation
Ala 273	Trp 274	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70263.4
Non-Polymers*	Number of molecules	6
	Total formula weight	694.8
All*	Total formula weight	70958.1

*Water molecules are not included.