6V4X

Cryo-EM structure of an active human histone pre-mRNA 3'-end processing machinery at 3.2 Angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Small nuclear ribonucleoprotein Sm D3	polymer	146	16111.7	1	UniProt (P62318) Pfam (PF01423)	Homo sapiens (Human)	Sm-D3,snRNP core protein D3
2	B (B)	Small nuclear ribonucleoprotein-associated proteins B and B'	polymer	95	10911.9	1	UniProt (P14678) Pfam (PF01423)	Homo sapiens (Human)	snRNP-B,Sm protein B/B',SmB/B'
3	C (F)	Small nuclear ribonucleoprotein F	polymer	86	9734.2	1	UniProt (P62306) Pfam (PF01423)	Homo sapiens (Human)	snRNP-F,Sm protein F,SmF
4	D (E)	Small nuclear ribonucleoprotein E	polymer	92	10817.6	1	UniProt (P62304) Pfam (PF01423)	Homo sapiens (Human)	snRNP-E,Sm protein E,SmE
5	E (G)	Small nuclear ribonucleoprotein G	polymer	84	9579.2	1	UniProt (P62308) Pfam (PF01423)	Homo sapiens (Human)	snRNP-G,Sm protein G,SmG
6	F (C)	U7 snRNA-associated Sm-like protein LSm10	polymer	123	14102.1	1	UniProt (Q969L4) Pfam (PF01423)	Homo sapiens (Human)
7	G (D)	U7 snRNA-associated Sm-like protein LSm11	polymer	259	28609.8	1	UniProt (P83369)	Homo sapiens (Human)
8	H (H)	Cleavage and polyadenylation specificity factor subunit 3	polymer	684	77580.9	1	UniProt (Q9UKF6) Pfam (PF11718) Pfam (PF00753) Pfam (PF10996) Pfam (PF07521)	Homo sapiens (Human)	Cleavage and polyadenylation specificity factor 73 kDa subunit,CPSF 73 kDa subunit,mRNA 3'-end-processing endonuclease CPSF-73
9	I (I)	Cleavage and polyadenylation specificity factor subunit 2	polymer	782	88597.7	1	UniProt (Q9P2I0) Pfam (PF16661) Pfam (PF10996) Pfam (PF07521) Pfam (PF13299)	Homo sapiens (Human)	Cleavage and polyadenylation specificity factor 100 kDa subunit,CPSF 100 kDa subunit
10	J (J)	Symplekin	polymer	1072	120355.1	1	UniProt (Q92797) Pfam (PF11935) Pfam (PF12295)	Homo sapiens (Human)
11	K (Z)	U7 snRNA	polymer	60	19097.2	1	GenBank (1633547)	Homo sapiens
12	L (Y)	modified H2a pre-mRNA	polymer	52	16627.0	1		Mus musculus
13	M, N (H)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 1 - 126 (UniProt: P62318)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

G: 1 - 76 (UniProt: P62308)

PDB	External Database	Details
Leu 77	-	expression tag
Glu 78	-	expression tag
His 79	-	expression tag
His 80	-	expression tag
His 81	-	expression tag
His 82	-	expression tag
His 83	-	expression tag
His 84	-	expression tag

D: 1 - 322 (UniProt: P83369)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

D: 323 - 360 (UniProt: P83369)

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	422124.4
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	422255.2

*Water molecules are not included.