6V2W

Crystal structure of the BRAF:MEK1 kinases in complex with AMPPNP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Serine/threonine-protein kinase B-raf	polymer	283	32098.1	1	UniProt (P15056) Pfam (PF07714) In PDB	Homo sapiens (Human)	Proto-oncogene B-Raf,p94,v-Raf murine sarcoma viral oncogene homolog B1
2	B	Dual specificity mitogen-activated protein kinase kinase 1	polymer	397	43790.3	1	UniProt (Q02750) Pfam (PF00069) In PDB	Homo sapiens (Human)	MKK1,ERK activator kinase 1,MAPK/ERK kinase 1,MEK 1
3	A, B	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
4	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A: 445 - 723 (UniProt: P15056)

PDB	External Database	Details
Gly 441	-	expression tag
Gly 442	-	expression tag
Gly 443	-	expression tag
Arg 444	-	expression tag

B: 1 - 393 (UniProt: Q02750)

PDB	External Database	Details
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Ala 218	Ser 218	engineered mutation
Ala 222	Ser 222	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	75888.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1061.0
All*	Total formula weight	76949.4

*Water molecules are not included.