6V29
Complex of double mutant (T89V,K162T) of E. coli L-asparaginase II with L-Asp
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | L-asparaginase 2 | polymer | 333 | 35556.8 | 4 | UniProt (P00805) Pfam (PF00710) Pfam (PF17763) In PDB | Escherichia coli (strain K12) | L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II |
2 | A, B, C, D | ASPARTIC ACID | non-polymer | 133.1 | 4 | Chemie (ASP) | |||
3 | A, B, C, D | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
4 | water | water | 18.0 | 1018 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 326 (UniProt: P00805)
PDB | External Database | Details |
---|---|---|
Met -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
Val 89 | Thr 111 | engineered mutation |
Thr 162 | Lys 184 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 142227.3 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 1085.0 | |
All* | Total formula weight | 143312.3 |