6V28

Complex of double mutant (T89V,K162T) of E. coli L-asparaginase II with L-Asp

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	L-asparaginase 2	polymer	333	35671.9	4	UniProt (P00805) Pfam (PF00710) Pfam (PF17763)	Escherichia coli (strain K12)	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	E, M (A, D)	IMIDAZOLE	non-polymer		69.1	2	Chemie (IMD)
3	F, G, H, I, J... (B, C, D)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
4	N, O, P, Q (A, B, C, D)	water	water		18.0	1223	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
Met -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Aei 12	Thr 34	modified residue
Val 89	Thr 111	engineered mutation
Thr 162	Lys 184	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	142687.7
Non-Polymers*	Number of molecules	9
	Total formula weight	782.8
All*	Total formula weight	143470.5

*Water molecules are not included.