6V28
Complex of double mutant (T89V,K162T) of E. coli L-asparaginase II with L-Asp
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004067 | molecular_function | asparaginase activity |
| A | 0006528 | biological_process | asparagine metabolic process |
| A | 0006530 | biological_process | asparagine catabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| A | 0032991 | cellular_component | protein-containing complex |
| A | 0042597 | cellular_component | periplasmic space |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0051289 | biological_process | protein homotetramerization |
| B | 0004067 | molecular_function | asparaginase activity |
| B | 0006528 | biological_process | asparagine metabolic process |
| B | 0006530 | biological_process | asparagine catabolic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| B | 0032991 | cellular_component | protein-containing complex |
| B | 0042597 | cellular_component | periplasmic space |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0051289 | biological_process | protein homotetramerization |
| C | 0004067 | molecular_function | asparaginase activity |
| C | 0006528 | biological_process | asparagine metabolic process |
| C | 0006530 | biological_process | asparagine catabolic process |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| C | 0032991 | cellular_component | protein-containing complex |
| C | 0042597 | cellular_component | periplasmic space |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0051289 | biological_process | protein homotetramerization |
| D | 0004067 | molecular_function | asparaginase activity |
| D | 0006528 | biological_process | asparagine metabolic process |
| D | 0006530 | biological_process | asparagine catabolic process |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| D | 0032991 | cellular_component | protein-containing complex |
| D | 0042597 | cellular_component | periplasmic space |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0051289 | biological_process | protein homotetramerization |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | binding site for residue IMD A 401 |
| Chain | Residue |
| A | ASN3 |
| A | ILE4 |
| A | THR5 |
| A | ASN47 |
| A | LYS49 |
| A | LYS79 |
| A | THR80 |
| site_id | AC2 |
| Number of Residues | 12 |
| Details | binding site for residue GOL B 901 |
| Chain | Residue |
| B | GLY10 |
| B | GLY11 |
| B | ALA14 |
| B | GLY28 |
| B | VAL30 |
| B | GLY31 |
| B | VAL32 |
| B | GLN52 |
| B | ASN55 |
| B | HOH1042 |
| B | ALA8 |
| B | THR9 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | binding site for residue GOL C 901 |
| Chain | Residue |
| C | ASP286 |
| C | ALA287 |
| C | GLY290 |
| C | PHE291 |
| C | PRO316 |
| C | GLN317 |
| C | GLN320 |
| C | HOH1012 |
| C | HOH1081 |
| site_id | AC4 |
| Number of Residues | 11 |
| Details | binding site for residue GOL C 902 |
| Chain | Residue |
| C | ASN209 |
| C | ASN209 |
| C | HOH1250 |
| C | HOH1250 |
| D | PHE254 |
| D | PHE254 |
| D | ASP255 |
| D | ASP255 |
| D | TYR289 |
| D | TYR289 |
| D | HOH670 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | binding site for residue GOL D 401 |
| Chain | Residue |
| C | SER224 |
| C | LEU226 |
| D | TYR236 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue GOL D 402 |
| Chain | Residue |
| D | SER173 |
| D | GLY177 |
| D | TYR181 |
| D | HOH706 |
| site_id | AC7 |
| Number of Residues | 11 |
| Details | binding site for residue GOL D 403 |
| Chain | Residue |
| D | ALA8 |
| D | THR9 |
| D | GLY10 |
| D | GLY11 |
| D | ALA14 |
| D | VAL30 |
| D | GLY31 |
| D | VAL32 |
| D | GLN52 |
| D | ASN55 |
| D | HOH524 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | binding site for residue GOL D 404 |
| Chain | Residue |
| D | PHE291 |
| D | GLN317 |
| D | GLN320 |
| D | HOH513 |
| D | HOH661 |
| D | HOH749 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue IMD D 405 |
| Chain | Residue |
| D | ASN3 |
| D | ILE4 |
| D | THR5 |
| D | ASN47 |
| D | LYS79 |
| D | THR80 |
| site_id | AD1 |
| Number of Residues | 19 |
| Details | binding site for Di-peptide GLY B 11 and AEI B 12 |
| Chain | Residue |
| A | ASN248 |
| A | GLU283 |
| B | GLY10 |
| B | ILE13 |
| B | ALA14 |
| B | GLY15 |
| B | TYR25 |
| B | VAL27 |
| B | ILE56 |
| B | GLY57 |
| B | SER58 |
| B | GLN59 |
| B | GLY88 |
| B | VAL89 |
| B | ASP90 |
| B | ALA114 |
| B | ARG116 |
| B | GOL901 |
| B | HOH1109 |
| site_id | AD2 |
| Number of Residues | 19 |
| Details | binding site for Di-peptide AEI B 12 and ILE B 13 |
| Chain | Residue |
| B | GLY88 |
| B | VAL89 |
| B | ASP90 |
| B | ALA114 |
| B | ARG116 |
| B | SER118 |
| B | HOH1004 |
| B | HOH1109 |
| A | ASN248 |
| A | GLU283 |
| B | GLY10 |
| B | GLY11 |
| B | ALA14 |
| B | GLY15 |
| B | TYR25 |
| B | LEU35 |
| B | GLY57 |
| B | SER58 |
| B | GLN59 |
| site_id | AD3 |
| Number of Residues | 18 |
| Details | binding site for Di-peptide GLY C 11 and AEI C 12 |
| Chain | Residue |
| C | GLY10 |
| C | ILE13 |
| C | ALA14 |
| C | GLY15 |
| C | TYR25 |
| C | ILE56 |
| C | GLY57 |
| C | SER58 |
| C | GLN59 |
| C | GLY88 |
| C | VAL89 |
| C | ASP90 |
| C | ALA114 |
| C | ARG116 |
| C | PRO117 |
| C | HOH1170 |
| D | ASN248 |
| D | GLU283 |
| site_id | AD4 |
| Number of Residues | 19 |
| Details | binding site for Di-peptide AEI C 12 and ILE C 13 |
| Chain | Residue |
| C | GLY10 |
| C | GLY11 |
| C | ALA14 |
| C | GLY15 |
| C | TYR25 |
| C | LEU35 |
| C | GLY57 |
| C | SER58 |
| C | GLN59 |
| C | GLY88 |
| C | VAL89 |
| C | ASP90 |
| C | ALA114 |
| C | ARG116 |
| C | PRO117 |
| C | HOH1163 |
| C | HOH1170 |
| D | ASN248 |
| D | GLU283 |
| site_id | AD5 |
| Number of Residues | 18 |
| Details | binding site for Di-peptide GLY D 11 and AEI D 12 |
| Chain | Residue |
| C | ASN248 |
| C | GLU283 |
| D | GLY10 |
| D | ILE13 |
| D | ALA14 |
| D | GLY15 |
| D | TYR25 |
| D | VAL27 |
| D | ILE56 |
| D | GLY57 |
| D | SER58 |
| D | GLN59 |
| D | GLY88 |
| D | VAL89 |
| D | ASP90 |
| D | ALA114 |
| D | GOL403 |
| D | HOH674 |
| site_id | AD6 |
| Number of Residues | 18 |
| Details | binding site for Di-peptide AEI D 12 and ILE D 13 |
| Chain | Residue |
| C | ASN248 |
| C | GLU283 |
| D | GLY10 |
| D | GLY11 |
| D | ALA14 |
| D | GLY15 |
| D | TYR25 |
| D | VAL27 |
| D | LEU35 |
| D | GLY57 |
| D | SER58 |
| D | GLN59 |
| D | GLY88 |
| D | VAL89 |
| D | ASP90 |
| D | ALA114 |
| D | HOH596 |
| D | HOH674 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: O-isoaspartyl threonine intermediate => ECO:0000255|PROSITE-ProRule:PRU10099, ECO:0000255|PROSITE-ProRule:PRU10100, ECO:0000269|PubMed:12595697, ECO:0000269|PubMed:1906013, ECO:0000269|PubMed:8434007, ECO:0000269|PubMed:8706862 |
| Chain | Residue | Details |
| B | AEI12 | |
| C | AEI12 | |
| D | AEI12 | |
| A | AEI12 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:12595697, ECO:0000269|PubMed:8434007, ECO:0007744|PDB:1NNS, ECO:0007744|PDB:3ECA |
| Chain | Residue | Details |
| A | SER58 | |
| A | VAL89 | |
| B | SER58 | |
| B | VAL89 | |
| C | SER58 | |
| C | VAL89 | |
| D | SER58 | |
| D | VAL89 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 455 |
| Chain | Residue | Details |
| A | THR162 | proton acceptor, proton donor |
| A | GLU283 | electrostatic stabiliser |
| A | VAL89 | electrostatic stabiliser, increase basicity, proton acceptor, proton donor, proton relay |
| A | AEI12 | covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, proton acceptor, proton donor |
| A | TYR25 | electrostatic stabiliser, increase nucleophilicity, proton acceptor, proton donor, proton relay |
| A | ASP90 | electrostatic stabiliser, increase acidity, increase basicity, increase nucleophilicity, proton acceptor, proton donor |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 455 |
| Chain | Residue | Details |
| B | AEI12 | covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, proton acceptor, proton donor |
| B | TYR25 | electrostatic stabiliser, increase nucleophilicity, proton acceptor, proton donor, proton relay |
| B | ASP90 | electrostatic stabiliser, increase acidity, increase basicity, increase nucleophilicity, proton acceptor, proton donor |
| B | THR162 | proton acceptor, proton donor |
| B | GLU283 | electrostatic stabiliser |
| B | VAL89 | electrostatic stabiliser, increase basicity, proton acceptor, proton donor, proton relay |
| site_id | MCSA3 |
| Number of Residues | 6 |
| Details | M-CSA 455 |
| Chain | Residue | Details |
| C | AEI12 | covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, proton acceptor, proton donor |
| C | TYR25 | electrostatic stabiliser, increase nucleophilicity, proton acceptor, proton donor, proton relay |
| C | ASP90 | electrostatic stabiliser, increase acidity, increase basicity, increase nucleophilicity, proton acceptor, proton donor |
| C | VAL89 | electrostatic stabiliser, increase basicity, proton acceptor, proton donor, proton relay |
| C | THR162 | proton acceptor, proton donor |
| C | GLU283 | electrostatic stabiliser |
| site_id | MCSA4 |
| Number of Residues | 6 |
| Details | M-CSA 455 |
| Chain | Residue | Details |
| D | AEI12 | covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, proton acceptor, proton donor |
| D | TYR25 | electrostatic stabiliser, increase nucleophilicity, proton acceptor, proton donor, proton relay |
| D | VAL89 | electrostatic stabiliser, increase basicity, proton acceptor, proton donor, proton relay |
| D | ASP90 | electrostatic stabiliser, increase acidity, increase basicity, increase nucleophilicity, proton acceptor, proton donor |
| D | THR162 | proton acceptor, proton donor |
| D | GLU283 | electrostatic stabiliser |
