6V25

Complex of mutant (K162M) of E. coli L-asparaginase II with L-Asp

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	L-asparaginase 2	polymer	333	35588.9	2	UniProt (P00805) Pfam (PF00710) Pfam (PF17763)	Escherichia coli (strain K12)	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	B, D (C, D)	L-asparaginase 2	polymer	333	35704.0	2	UniProt (P00805) Pfam (PF00710) Pfam (PF17763)	Escherichia coli (strain K12)	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
3	E, F (A, B)	ASPARTIC ACID	non-polymer		133.1	2	Chemie (ASP)
4	G, H, I, J (A, C, B, D)	water	water		18.0	1456	Chemie (HOH)

Sequence modifications

A, B: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
Met -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Met 162	Lys 184	engineered mutation

C, D: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
Met -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Aei 12	Thr 34	modified residue
Met 162	Lys 184	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	142585.8
Non-Polymers*	Number of molecules	2
	Total formula weight	266.2
All*	Total formula weight	142852.0

*Water molecules are not included.