6UZX

Crystal structure of GLUN1/GLUN2A-4M mutant ligand-binding domain in complex with glycine and UBP791

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glutamate receptor ionotropic, NMDA 1	polymer	292	33340.0	1	UniProt (P35439) Pfam (PF10613) Pfam (PF00060)	Rattus norvegicus (Rat)	GluN1,Glutamate [NMDA] receptor subunit zeta-1,N-methyl-D-aspartate receptor subunit NR1,NMD-R1
2	B (B)	Glutamate receptor ionotropic, NMDA 2A	polymer	282	31858.5	1	UniProt (Q00959) Pfam (PF10613) Pfam (PF00060)	Rattus norvegicus (Rat)	GluN2A,Glutamate [NMDA] receptor subunit epsilon-1,N-methyl D-aspartate receptor subtype 2A,NR2A
3	C, E (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	D (A)	GLYCINE	non-polymer		75.1	1	Chemie (GLY)
5	F (B)	(2S,3R)-1-[7-(2-carboxyethyl)phenanthrene-2-carbonyl]piperazine-2,3-dicarboxylic acid	non-polymer		450.4	1	Chemie (QM4)
6	G, H (A, B)	water	water		18.0	115	Chemie (HOH)

Sequence modifications

A: 2 - 152 (UniProt: P35439)

PDB	External Database	Details
Gly 1	-	expression tag

A: 155 - 292 (UniProt: P35439)

PDB	External Database	Details
Gly 153	-	linker
Thr 154	-	linker

B: 5 - 142 (UniProt: Q00959)

PDB	External Database	Details
Arg 17	Ala 414	engineered mutation

B: 145 - 286 (UniProt: Q00959)

PDB	External Database	Details
Gly 143	-	linker
Thr 144	-	linker
Met 222	Lys 738	engineered mutation
Arg 224	Gly 740	engineered mutation
Lys 225	Arg 741	engineered mutation
Thr 242	Ser 758	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	65198.5
Non-Polymers*	Number of molecules	4
	Total formula weight	709.7
All*	Total formula weight	65908.2

*Water molecules are not included.