6UZX
Crystal structure of GLUN1/GLUN2A-4M mutant ligand-binding domain in complex with glycine and UBP791
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-23 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9201 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.987, 85.081, 120.412 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.150 - 2.410 |
R-factor | 0.1941 |
Rwork | 0.191 |
R-free | 0.24530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nf6 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.478 |
Phasing software | REFMAC |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.150 | 2.490 |
High resolution limit [Å] | 2.410 | 2.410 |
Rmerge | 0.156 | 0.668 |
Number of reflections | 23716 | 1995 |
<I/σ(I)> | 11.6 | |
Completeness [%] | 98.0 | |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 100 mM HEPES, pH 7.0, 75 mM sodium chloride, 18% PEG2000 MME |