6UU9
E. coli mutant sigma-S transcription initiation complex with an 8-nt RNA ("Fresh" mutant crystal soaked with GTP, UTP, CTP, and ddATP for 30 minutes)
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (AAA, BBB) | DNA-directed RNA polymerase subunit alpha | polymer | 242 | 26899.6 | 2 | UniProt (A0A377D9Q8) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | |
| 2 | C (CCC) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (DDD) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli | RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta' |
| 4 | E (EEE) | DNA-directed RNA polymerase subunit omega | polymer | 90 | 10118.4 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli | RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega |
| 5 | F (FFF) | RNA polymerase sigma factor RpoS | polymer | 336 | 38705.5 | 1 | UniProt (A0A377K1M2) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P13445) | Escherichia coli | Sigma S,Sigma-38 |
| 6 | G (111) | Synthetic DNA 50-mer (promoter non-template strand) | polymer | 50 | 15313.8 | 1 | synthetic construct | ||
| 7 | H (222) | Synthetic DNA 50-mer (promoter template strand) | polymer | 50 | 15563.0 | 1 | synthetic construct | ||
| 8 | I (333) | RNA 8-mer | polymer | 8 | 2694.5 | 1 | synthetic construct | ||
| 9 | J, K (DDD) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 10 | L, M (DDD) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 11 | N (DDD) | 2',3'-DIDEOXYADENOSINE-5'-MONOPHOSPHATE | non-polymer | 315.2 | 1 | Chemie (2DA) | |||
| 12 | O (DDD) | DIPHOSPHATE | non-polymer | 173.9 | 1 | Chemie (DPO) |
Sequence modifications
AAA, BBB: 1 - 235 (UniProt: A0A377D9Q8)
FFF: 1 - 328 (UniProt: A0A377K1M2)
| PDB | External Database | Details |
|---|---|---|
| Ala -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 2 | Ser 2 | conflict |
| Gly 219 | Ile 219 | engineered mutation |
| Ala 221 | Ser 221 | engineered mutation |
| Leu 329 | - | expression tag |
| Glu 330 | - | expression tag |
| His 331 | - | expression tag |
| His 332 | - | expression tag |
| His 333 | - | expression tag |
| His 334 | - | expression tag |
| His 335 | - | expression tag |
| His 336 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 442382.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 668.6 | |
| All* | Total formula weight | 443050.6 |
