6UU9
E. coli mutant sigma-S transcription initiation complex with an 8-nt RNA ("Fresh" mutant crystal soaked with GTP, UTP, CTP, and ddATP for 30 minutes)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97910 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 132.184, 153.474, 230.825 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.988 - 5.400 |
Rwork | 0.314 |
R-free | 0.36220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ipl |
RMSD bond length | 0.006 |
RMSD bond angle | 1.383 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 5.720 |
High resolution limit [Å] | 5.395 | 5.400 |
Number of reflections | 16580 | 2511 |
<I/σ(I)> | 6.93 | 0.47 |
Completeness [%] | 99.0 | 95.4 |
Redundancy | 5.9 | 5.8 |
CC(1/2) | 0.995 | 0.157 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | PEG3350, sodium chloride, HEPES |