6UQB

Crystal structure of R471A variant of cytosolic fumarate hydratase from Leishmania major in a complex with S-malate and malonate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Fumarate hydratase 2	polymer	604	66427.4	2	UniProt (E9AE57) Pfam (PF05681) Pfam (PF05683)	Leishmania major strain Friedlin	LmFH-2
2	C, H (A, B)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
3	D, I (A, B)	(2S)-2-hydroxybutanedioic acid	non-polymer		134.1	2	Chemie (LMR)
4	E, F, J, K (A, B)	MALONIC ACID	non-polymer		104.1	4	Chemie (MLA)
5	G, L (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6	M, N (A, B)	water	water		18.0	917	Chemie (HOH)

Sequence modifications

A, B: 1 - 568 (UniProt: E9AE57)

PDB	External Database	Details
Met -35	-	expression tag
Gly -34	-	expression tag
Ser -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Leu -22	-	expression tag
Val -21	-	expression tag
Pro -20	-	expression tag
Arg -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
Met -15	-	expression tag
Ala -14	-	expression tag
Ser -13	-	expression tag
Met -12	-	expression tag
Thr -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Gln -8	-	expression tag
Gln -7	-	expression tag
Met -6	-	expression tag
Gly -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Ala 471	Arg 471	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	132854.7
Non-Polymers*	Number of molecules	10
	Total formula weight	1571.9
All*	Total formula weight	134426.6

*Water molecules are not included.