6UPH

Structure of a Yeast Centromeric Nucleosome at 2.7 Angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3-like centromeric protein CSE4	polymer	229	26885.4	2	UniProt (P36012) Pfam (PF00125) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	CENP-A homolog,Chromosome segregation protein 4
2	B, F	Histone H4	polymer	118	13332.4	2	UniProt (Q6CMU6) Pfam (PF15511) In PDB	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast)
3	C, G	Histone H2A	polymer	145	15739.0	2	UniProt (Q6CK59) Pfam (PF00125) Pfam (PF16211) In PDB	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast)
4	D, H	Histone H2B.1	polymer	147	16219.4	2	UniProt (Q6CK60) Pfam (PF00125) In PDB	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast)
5	I	DNA (119-MER)	polymer	147	45138.8	1		unidentified
6	J	DNA (119-MER)	polymer	147	45610.0	1		unidentified

Sequence modifications

B, F: 1 - 103 (UniProt: Q6CMU6)

PDB	External Database	Details
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

C, G: 1 - 130 (UniProt: Q6CK59)

PDB	External Database	Details
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

D, H: 1 - 132 (UniProt: Q6CK60)

PDB	External Database	Details
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	235101.3
All*	Total formula weight	235101.3

*Water molecules are not included.