6UMX

Structural basis for specific inhibition of extracellular activation of pro/latent myostatin by SRK-015

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (A, B)	Growth/differentiation factor 8	polymer	365	41642.3	2	UniProt (O14793) Pfam (PF00688) Pfam (PF00019)	Homo sapiens (Human)	GDF-8, Myostatin
2	B, D (L, l)	GL29H4-16 Fab Light Chain,GL29H4-16 Fab Light Chain	polymer	215	22655.9	2	UniProt (P0DOY2) Pfam (PF07654)	Homo sapiens (Human)
3	C, E (H, h)	GL29H4-16 Fab Heavy Chain,GL29H4-16 Fab Heavy Chain	polymer	229	24725.9	2	UniProt (Q5EFE5) Pfam (PF07686) Pfam (PF07654)	Homo sapiens (Human)
4	G (h)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	H, I (H, l)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A, B: 24 - 375 (UniProt: O14793)

PDB	External Database	Details
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
Glu 17	-	expression tag
Asn 18	-	expression tag
Leu 19	-	expression tag
Tyr 20	-	expression tag
Phe 21	-	expression tag
Gln 22	-	expression tag
Ser 23	-	expression tag
Ala 99	Asp 99	engineered mutation
Ala 263	Arg 263	engineered mutation
Ala 266	Arg 266	engineered mutation

L, l: 1 - 110 (PDB: 6UMX)
L, l: 111 - 215 (UniProt: P0DOY2)
H, h: 1 - 121 (PDB: 6UMX)
H, h: 122 - 229 (UniProt: Q5EFE5)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	178048.2
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	178140.3

*Water molecules are not included.