6UKL

Crystal Structure of a DiB2-split Protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, C, E)	Outer membrane lipoprotein Blc	polymer	107	12298.8	3	UniProt (P0A902) Pfam (PF08212) UniProt (by SIFTS) (P0A901)	Escherichia coli
2	D, E, F (D, F, B)	Outer membrane lipoprotein Blc	polymer	69	7903.0	3	UniProt (P0A902) Pfam (PF08212) UniProt (by SIFTS) (P0A901)	Escherichia coli
3	G, H, I, J, K... (A, C, E, D, B)	SULFATE ION	non-polymer		96.1	15	Chemie (SO4)
4	O, U (C, D)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
5	R (E)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
6	AA, BA, CA, DA, Y... (E, D, F, B, A...)	water	water		18.0	106	Chemie (HOH)

Sequence modifications

A, C, E: 23 - 109 (UniProt: P0A902)

PDB	External Database	Details
Met 3	-	expression tag
Gly 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Leu 11	-	expression tag
Glu 12	-	expression tag
Ser 13	-	expression tag
Thr 14	-	expression tag
Ser 15	-	expression tag
Leu 16	-	expression tag
Tyr 17	-	expression tag
Lys 18	-	expression tag
Lys 19	-	expression tag
Ser 20	-	expression tag
Ser 21	-	expression tag
Ser 22	-	expression tag
Cys 36	Ala 36	engineered mutation

D, F, B: 110 - 177 (UniProt: P0A902)

PDB	External Database	Details
Met 109	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	60605.2
Non-Polymers*	Number of molecules	18
	Total formula weight	2447.9
All*	Total formula weight	63053.1

*Water molecules are not included.