6UKL
Crystal Structure of a DiB2-split Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-11-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.241, 68.241, 216.292 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.100 - 2.020 |
| R-factor | 0.2071 |
| Rwork | 0.205 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qwd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.689 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.100 | 2.050 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmeas | 0.099 | 0.989 |
| Rpim | 0.033 | 0.335 |
| Total number of observations | 346459 | |
| Number of reflections | 39425 | 1913 |
| <I/σ(I)> | 11.5 | 2.1 |
| Completeness [%] | 100.0 | 97.5 |
| Redundancy | 8.8 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | 1.6 M ammonium sulfate, 0.1 M MES, pH 4.5, supplemented with 10% 0.1 M iron(III) chloride hexahydrate |
