6UFP

Structure of proline utilization A with the FAD covalently modified by L-thiazolidine-2-carboxylate and three cysteines (Cys46, Cys470, Cys638) modified to S,S-(2-HYDROXYETHYL)THIOCYSTEINE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Bifunctional protein PutA	polymer	1235	132113.9	1	UniProt (F7X6I3) Pfam (PF18327) Pfam (PF14850) Pfam (PF01619) Pfam (PF00171) In PDB	Sinorhizobium meliloti (strain SM11)
2	B	Bifunctional protein PutA	polymer	1235	132190.0	1	UniProt (F7X6I3) Pfam (PF18327) Pfam (PF14850) Pfam (PF01619) Pfam (PF00171) In PDB	Sinorhizobium meliloti (strain SM11)
3	A, B	DIHYDROFLAVINE-ADENINE DINUCLEOTIDE	non-polymer		787.6	2	Chemie (FDA)
4	A, B	(2S)-1,3-thiazolidine-2-carboxylic acid	non-polymer		133.2	2	Chemie (T2C)
5	A, B	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
6	A, B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
7	A, B	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
8	A, B	FORMIC ACID	non-polymer		46.0	2	Chemie (FMT)
9		water	water		18.0	1219	Chemie (HOH)

Sequence modifications

A: 1 - 1233 (UniProt: F7X6I3)

PDB	External Database	Details
Ser -1	-	expression tag
Met 0	-	expression tag

B: 1 - 1233 (UniProt: F7X6I3)

PDB	External Database	Details
Ser -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	264303.9
Non-Polymers*	Number of molecules	16
	Total formula weight	4049.0
All*	Total formula weight	268352.9

*Water molecules are not included.