6UC3

Spectroscopic and structural characterization of a genetically encoded direct sensor for protein-ligand interactions

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Streptavidin	polymer	136	14641.8	2	UniProt (P22629) Pfam (PF01382) In PDB	Streptomyces avidinii
2	A, B	BIOTIN	non-polymer		244.3	2	Chemie (BTN)
3		water	water		18.0	133	Chemie (HOH)

Sequence modifications

A, B: 13 - 139 (UniProt: P22629)

PDB	External Database	Details
Met 12	-	initiating methionine
Dv7 112	Ser 136	engineered mutation
Leu 140	-	expression tag
Glu 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	29283.7
Non-Polymers*	Number of molecules	2
	Total formula weight	488.6
All*	Total formula weight	29772.3

*Water molecules are not included.