6UB2

Crystal structure of a GH128 (subgroup IV) endo-beta-1,3-glucanase from Lentinula edodes (LeGH128_IV)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Endo-beta-1,3-glucanase	polymer	270	28780.8	4	UniProt (G9M5R4) Pfam (PF11790)	Lentinula edodes (Shiitake mushroom)
2	E, G, J, L (A, B, C, D)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	4	Chemie (MES)
3	F, I, K, O (A, B, C, D)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
4	H, M, N (B, D)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
5	P, Q, R, S (A, B, C, D)	water	water		18.0	1271	Chemie (HOH)

Sequence modifications

A, B, C, D: 21 - 267 (UniProt: G9M5R4)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Ser 8	-	expression tag
Ser 9	-	expression tag
Gly 10	-	expression tag
Leu 11	-	expression tag
Val 12	-	expression tag
Pro 13	-	expression tag
Arg 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
Met 18	-	expression tag
Ala 19	-	expression tag
Ser 20	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	115123.3
Non-Polymers*	Number of molecules	11
	Total formula weight	1210.9
All*	Total formula weight	116334.3

*Water molecules are not included.