6UB2
Crystal structure of a GH128 (subgroup IV) endo-beta-1,3-glucanase from Lentinula edodes (LeGH128_IV)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-10 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.817, 75.159, 99.450 |
Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
Resolution | 49.090 - 1.800 |
R-factor | 0.1744 |
Rwork | 0.172 |
R-free | 0.21250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.769 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.090 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 94945 | 15156 |
<I/σ(I)> | 9.05 | |
Completeness [%] | 98.2 | |
Redundancy | 6.9 | |
CC(1/2) | 0.996 | 0.628 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 1.6 M ammonium sulfate 0.1 M MES 0% dioxane |