6UB2

Crystal structure of a GH128 (subgroup IV) endo-beta-1,3-glucanase from Lentinula edodes (LeGH128_IV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	72.817, 75.159, 99.450
Unit cell angles	90.00, 103.60, 90.00

Refinement procedure

Resolution	49.090 - 1.800
R-factor	0.1744
Rwork	0.172
R-free	0.21250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.769
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.090	1.910
High resolution limit [Å]	1.800	1.800
Number of reflections	94945	15156
<I/σ(I)>	9.05
Completeness [%]	98.2
Redundancy	6.9
CC(1/2)	0.996	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	1.6 M ammonium sulfate 0.1 M MES 0% dioxane