6UB1

Crystal structure of a GH128 (subgroup III) curdlan-specific exo-beta-1,3-glucanase from Blastomyces gilchristii (BgGH128_III) in complex with laminaribiose at -3 and -2 subsites

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	GLYCOSIDE HYDROLASE	polymer	261	27993.1	4	UniProt (A0A179UGT5) Pfam (PF11790)	Blastomyces gilchristii
2	E, F, G (E, F, G)	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose	branched		342.3	3	In PDB BIRD (PRD_900024) GlyTouCan (G36535HU)		beta-laminaribiose
3	H (B)	beta-D-glucopyranose	non-polymer		180.2	1	Chemie (BGC)
4	I, J, K, L (A, B, C, D)	water	water		18.0	1096	Chemie (HOH)

Sequence modifications

A, B, C, D: 23 - 262 (UniProt: A0A179UGT5)

PDB	External Database	Details
Met 2	-	initiating methionine
Gly 3	-	expression tag
Ser 4	-	expression tag
Ser 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
Ser 12	-	expression tag
Ser 13	-	expression tag
Gly 14	-	expression tag
Leu 15	-	expression tag
Val 16	-	expression tag
Pro 17	-	expression tag
Ala 18	-	expression tag
Gly 19	-	expression tag
Ser 20	-	expression tag
His 21	-	expression tag
Met 22	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	111972.5
Branched	Number of molecules	3
	Total formula weight	1026.9
Non-Polymers*	Number of molecules	1
	Total formula weight	180.2
All*	Total formula weight	113179.6

*Water molecules are not included.