6UAS
Crystal structure of a GH128 (subgroup I) endo-beta-1,3-glucanase (E199A mutant) from Amycolatopsis mediterranei (AmGH128_I) in complex with laminaripentaose
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Glycoside Hydrolase | polymer | 263 | 27859.9 | 1 | UniProt (G0FQ07) In PDB | Amycolatopsis mediterranei | |
2 | B | beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-alpha-D-glucopyranose | branched | 828.7 | 1 | In PDB GlyTouCan (G20125LZ) | |||
3 | A | beta-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (BGC) | |||
4 | A | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
5 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
6 | water | water | 18.0 | 218 | Chemie (HOH) |
Sequence modifications
A: 28 - 269 (UniProt: G0FQ07)
PDB | External Database | Details |
---|---|---|
Met 7 | - | initiating methionine |
Gly 8 | - | expression tag |
Ser 9 | - | expression tag |
Ser 10 | - | expression tag |
His 11 | - | expression tag |
His 12 | - | expression tag |
His 13 | - | expression tag |
His 14 | - | expression tag |
His 15 | - | expression tag |
His 16 | - | expression tag |
Ser 17 | - | expression tag |
Ser 18 | - | expression tag |
Gly 19 | - | expression tag |
Leu 20 | - | expression tag |
Val 21 | - | expression tag |
Pro 22 | - | expression tag |
Ala 23 | - | expression tag |
Gly 24 | - | expression tag |
Ser 25 | - | expression tag |
His 26 | - | expression tag |
Met 27 | - | expression tag |
Ala 199 | Glu 199 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 27859.9 | |
Branched | Number of molecules | 1 |
Total formula weight | 828.7 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 351.7 | |
All* | Total formula weight | 29040.3 |