6UAS

Crystal structure of a GH128 (subgroup I) endo-beta-1,3-glucanase (E199A mutant) from Amycolatopsis mediterranei (AmGH128_I) in complex with laminaripentaose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glycoside Hydrolase	polymer	263	27859.9	1	UniProt (G0FQ07)	Amycolatopsis mediterranei
2	B (B)	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-alpha-D-glucopyranose	branched		828.7	1	In PDB GlyTouCan (G20125LZ)
3	C (A)	beta-D-glucopyranose	non-polymer		180.2	1	Chemie (BGC)
4	D (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	E (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
6	F (A)	water	water		18.0	218	Chemie (HOH)

Sequence modifications

A: 28 - 269 (UniProt: G0FQ07)

PDB	External Database	Details
Met 7	-	initiating methionine
Gly 8	-	expression tag
Ser 9	-	expression tag
Ser 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
Ser 17	-	expression tag
Ser 18	-	expression tag
Gly 19	-	expression tag
Leu 20	-	expression tag
Val 21	-	expression tag
Pro 22	-	expression tag
Ala 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag
His 26	-	expression tag
Met 27	-	expression tag
Ala 199	Glu 199	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27859.9
Branched	Number of molecules	1
	Total formula weight	828.7
Non-Polymers*	Number of molecules	3
	Total formula weight	351.7
All*	Total formula weight	29040.3

*Water molecules are not included.