6U62

Raptor-Rag-Ragulator complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Regulatory-associated protein of mTOR	polymer	1392	154915.8	1	UniProt (Q8N122) Pfam (PF14538) Pfam (PF02985) Pfam (PF00400)	Homo sapiens (Human)	Raptor,p150 target of rapamycin (TOR)-scaffold protein
2	B (B)	Ras-related GTP-binding protein A	polymer	313	36615.2	1	UniProt (Q7L523) Pfam (PF04670)	Homo sapiens (Human)	RagA,Adenovirus E3 14.7 kDa-interacting protein 1,FIP-1
3	C (C)	Ras-related GTP-binding protein C	polymer	400	44334.8	1	UniProt (Q9HB90) Pfam (PF04670)	Homo sapiens (Human)	RagC,GTPase-interacting protein 2,TIB929
4	D (D)	Ragulator complex protein LAMTOR1	polymer	158	17359.2	1	UniProt (Q6IAA8) Pfam (PF15454)	Homo sapiens (Human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1,Lipid raft adaptor protein p18,Protein associated with DRMs and endosomes,p27Kip1-releasing factor from RhoA,p27RF-Rho
5	E (E)	Ragulator complex protein LAMTOR2	polymer	125	13517.5	1	UniProt (Q9Y2Q5) Pfam (PF03259)	Homo sapiens (Human)	Endosomal adaptor protein p14,Late endosomal/lysosomal Mp1-interacting protein,Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2,Mitogen-activated protein-binding protein-interacting protein,MAPBP-interacting protein,Roadblock domain-containing protein 3
6	F (F)	Ragulator complex protein LAMTOR3	polymer	162	17579.8	1	UniProt (Q9UHA4) Pfam (PF08923)	Homo sapiens (Human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3,MEK-binding partner 1,Mp1,Mitogen-activated protein kinase kinase 1-interacting protein 1,Mitogen-activated protein kinase scaffold protein 1
7	G (G)	Ragulator complex protein LAMTOR4	polymer	100	10776.2	1	UniProt (Q0VGL1)	Homo sapiens (Human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4
8	H (H)	Ragulator complex protein LAMTOR5	polymer	91	9622.9	1	UniProt (O43504) Pfam (PF16672)	Homo sapiens (Human)	Hepatitis B virus X-interacting protein,HBX-interacting protein,Late endosomal/lysosomal adaptor and MAPK and MTOR activator 5
9	I (B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
10	J (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
11	K (C)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)

Sequence modifications

A: 1 - 1335 (UniProt: Q8N122)

PDB	External Database	Details
Gly 1336	-	expression tag
Gly 1337	-	expression tag
Gly 1338	-	expression tag
Ser 1339	-	expression tag
Leu 1340	-	expression tag
Glu 1341	-	expression tag
Val 1342	-	expression tag
Leu 1343	-	expression tag
Phe 1344	-	expression tag
Gln 1345	-	expression tag
Gly 1346	-	expression tag
Pro 1347	-	expression tag
Gly 1348	-	expression tag
Ser 1349	-	expression tag
Gly 1350	-	expression tag
Gly 1351	-	expression tag
Gly 1352	-	expression tag
Ser 1353	-	expression tag
Asp 1354	-	expression tag
Tyr 1355	-	expression tag
Lys 1356	-	expression tag
Asp 1357	-	expression tag
Asp 1358	-	expression tag
Asp 1359	-	expression tag
Asp 1360	-	expression tag
Lys 1361	-	expression tag
Ser 1362	-	expression tag
Ser 1363	-	expression tag
Gly 1364	-	expression tag
Trp 1365	-	expression tag
Ser 1366	-	expression tag
His 1367	-	expression tag
Pro 1368	-	expression tag
Gln 1369	-	expression tag
Phe 1370	-	expression tag
Glu 1371	-	expression tag
Lys 1372	-	expression tag
Gly 1373	-	expression tag
Gly 1374	-	expression tag
Gly 1375	-	expression tag
Ala 1376	-	expression tag
Arg 1377	-	expression tag
Gly 1378	-	expression tag
Gly 1379	-	expression tag
Ser 1380	-	expression tag
Gly 1381	-	expression tag
Gly 1382	-	expression tag
Gly 1383	-	expression tag
Ser 1384	-	expression tag
Trp 1385	-	expression tag
Ser 1386	-	expression tag
His 1387	-	expression tag
Pro 1388	-	expression tag
Gln 1389	-	expression tag
Phe 1390	-	expression tag
Glu 1391	-	expression tag
Lys 1392	-	expression tag

C: 2 - 399 (UniProt: Q9HB90)

PDB	External Database	Details
Gly 0	-	expression tag
Pro 1	-	expression tag
Asn 75	Ser 75	engineered mutation
Asn 90	Thr 90	engineered mutation

D: 6 - 161 (UniProt: Q6IAA8)

PDB	External Database	Details
Gly 4	-	expression tag
Pro 5	-	expression tag

F: 1 - 124 (UniProt: Q9UHA4)

PDB	External Database	Details
Glu 125	-	expression tag
Asn 126	-	expression tag
Leu 127	-	expression tag
Tyr 128	-	expression tag
Phe 129	-	expression tag
Gln 130	-	expression tag
Gly 131	-	expression tag
Ser 132	-	expression tag
Ser 133	-	expression tag
Gly 134	-	expression tag
Trp 135	-	expression tag
Ser 136	-	expression tag
His 137	-	expression tag
Pro 138	-	expression tag
Gln 139	-	expression tag
Phe 140	-	expression tag
Glu 141	-	expression tag
Lys 142	-	expression tag
Gly 143	-	expression tag
Gly 144	-	expression tag
Gly 145	-	expression tag
Ser 146	-	expression tag
Gly 147	-	expression tag
Gly 148	-	expression tag
Gly 149	-	expression tag
Ser 150	-	expression tag
Gly 151	-	expression tag
Gly 152	-	expression tag
Gly 153	-	expression tag
Ser 154	-	expression tag
Trp 155	-	expression tag
Ser 156	-	expression tag
His 157	-	expression tag
Pro 158	-	expression tag
Gln 159	-	expression tag
Phe 160	-	expression tag
Glu 161	-	expression tag
Lys 162	-	expression tag

G: 2 - 99 (UniProt: Q0VGL1)

PDB	External Database	Details
Gly 0	-	expression tag
Pro 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	304721.4
Non-Polymers*	Number of molecules	3
	Total formula weight	990.7
All*	Total formula weight	305712.1

*Water molecules are not included.