6U43

Crystal structure of Methanoperedens nitroreducens elongation factor 2 H595N bound to GMPPCP and magnesium (triclinic crystal form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Elongation factor 2	polymer	753	83304.2	1	UniProt (A0A062V290)	Candidatus Methanoperedens nitroreducens	EF-2
2	B (A)	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER	non-polymer		521.2	1	Chemie (GCP)
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	D, F (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	E (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	G (A)	water	water		18.0	807	Chemie (HOH)

Sequence modifications

A: 1 - 729 (UniProt: A0A062V290)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
Ser -21	-	expression tag
Asp -20	-	expression tag
Lys -19	-	expression tag
Ile -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Asn 595	His 595	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	83304.2
Non-Polymers*	Number of molecules	5
	Total formula weight	773.1
All*	Total formula weight	84077.3

*Water molecules are not included.