6U43

Crystal structure of Methanoperedens nitroreducens elongation factor 2 H595N bound to GMPPCP and magnesium (triclinic crystal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 1
Unit cell lengths	57.195, 62.524, 63.396
Unit cell angles	64.21, 69.68, 80.51

Refinement procedure

Resolution	54.264 - 1.400
R-factor	0.1665
Rwork	0.165
R-free	0.19160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Saccharomyces cerevisiae EF-2 (PDB entry 1N0V)
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3211)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.300	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.044	0.545
Number of reflections	131884	17221
<I/σ(I)>	9.7	1.2
Completeness [%]	90.3
Redundancy	2.2
CC(1/2)	0.999	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	100 mM HEPES, pH 7.1-7.6, 200 mM ammonium sulfate, 5-15 % (v/v) isopropanol, 16.5-20 % (w/v) polyethylene glycol (PEG) 4000, 9 mM GppCp, and 15 mM MgCl2