6TWI

Crystal structure of the haemagglutinin mutant (Gln226Leu, Gly228Ser) from an H10N7 seal influenza virus isolated in Germany in complex with avian receptor analogue 3'-SLN

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, C, E)	Hemagglutinin	polymer	325	35514.1	3	UniProt (A0A0A7HR51)	Influenza A virus (A/harbour seal/Germany/1/2014(H10N7))
2	B, D, F (B, D, F)	Hemagglutinin HA2	polymer	177	20313.4	3	UniProt (A0A0A7HR51)	Influenza A virus (A/harbour seal/Germany/1/2014(H10N7))
3	G, H, J (G, H, J)	N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		674.6	3	In PDB BIRD (PRD_900067) GlyTouCan (G00065MO)		3'-sialyl-N-acetyllactosamine
4	I (I)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
5	K, O, Q, T, V (A, B, C, E, F)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)
6	L, M, P, R (A, B, C)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
7	N, S, U (A, C, E)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
8	AA, BA, W, X, Y... (E, F, A, B, C...)	water	water		18.0	497	Chemie (HOH)

Sequence modifications

A, C, E: 1 - 323 (UniProt: A0A0A7HR51)

PDB	External Database	Details
Asp -1	-	expression tag
Pro 0	-	expression tag
Ser 221	Gly 230	engineered mutation

B, D, F: 1 - 176 (UniProt: A0A0A7HR51)

PDB	External Database	Details
Lys 177	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	167482.6
Branched	Number of molecules	4
	Total formula weight	2448.2
Non-Polymers*	Number of molecules	12
	Total formula weight	1474.5
All*	Total formula weight	171405.3

*Water molecules are not included.