6TWI

Crystal structure of the haemagglutinin mutant (Gln226Leu, Gly228Ser) from an H10N7 seal influenza virus isolated in Germany in complex with avian receptor analogue 3'-SLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9798
Spacegroup name	P 1 21 1
Unit cell lengths	64.843, 215.101, 78.246
Unit cell angles	90.00, 96.78, 90.00

Refinement procedure

Resolution	73.080 - 2.270
R-factor	0.2466
Rwork	0.245
R-free	0.27270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d00
RMSD bond length	0.006
RMSD bond angle	1.000
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.080	2.310
High resolution limit [Å]	2.270	2.270
Number of reflections	97730	4834
<I/σ(I)>	5.2	1.4
Completeness [%]	99.9	99.9
Redundancy	3.3	3.2
CC(1/2)	0.987	0.436

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	6% PEG3350, 0.1 M MES pH6.5