6TRT

Chaetomium thermophilum UDP-Glucose Glucosyl Transferase (UGGT) double cysteine mutant S180C/T742C.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	UDP-glucose-glycoprotein glucosyltransferase-like protein	polymer	1494	169656.4	1	UniProt (G0SB58) Pfam (PF18400) Pfam (PF18401) Pfam (PF18402) Pfam (PF18403) Pfam (PF06427) Pfam (PF18404)	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
2	B, D (B, D)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
3	C (C)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	1	In PDB GlyTouCan (G00335DM)
4	E, F, G (A)	TERBIUM(III) ION	non-polymer		158.9	3	Chemie (TB)
5	H, I (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)

Sequence modifications

A: 24 - 1505 (UniProt: G0SB58)

PDB	External Database	Details
Glu 21	-	expression tag
Thr 22	-	expression tag
Gly 23	-	expression tag
Cys 180	Ser 180	engineered mutation
Cys 742	Thr 742	engineered mutation
Gly 1506	-	expression tag
Thr 1507	-	expression tag
Lys 1508	-	expression tag
His 1509	-	expression tag
His 1510	-	expression tag
His 1511	-	expression tag
His 1512	-	expression tag
His 1513	-	expression tag
His 1514	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	169656.4
Branched	Number of molecules	3
	Total formula weight	2570.3
Non-Polymers*	Number of molecules	5
	Total formula weight	919.2
All*	Total formula weight	173146.0

*Water molecules are not included.