6TIZ

DROSOPHILA GDP-TUBULIN Y222F MUTANT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Tubulin alpha-1 chain	polymer	450	49989.3	2	UniProt (P06603) Pfam (PF00091) Pfam (PF03953) In PDB	Drosophila melanogaster (Fruit fly)
2	B, D	Tubulin beta-1 chain	polymer	447	50178.1	2	UniProt (Q24560) Pfam (PF00091) Pfam (PF03953) In PDB	Drosophila melanogaster (Fruit fly)	Beta-1-tubulin
3	E	Stathmin-4	polymer	143	16851.1	1	UniProt (P63043) Pfam (PF00836) In PDB	Rattus norvegicus (Norway rat)	Stathmin-like protein B3,RB3
4	A, C	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
5	A, C	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	A, B, C, D, E	SULFATE ION	non-polymer		96.1	13	Chemie (SO4)
7	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
8	B, D	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
9		water	water		18.0	759	Chemie (HOH)

Sequence modifications

A, C: 1 - 450 (UniProt: P06603)

PDB	External Database	Details
Arg 40	Lys 40	engineered mutation

B, D: 1 - 447 (UniProt: Q24560)

PDB	External Database	Details
Phe 222	Tyr 222	engineered mutation

E: 4 - 145 (UniProt: P63043)

PDB	External Database	Details
Met 3	-	initiating methionine
Ala 4	Ser 48	engineered mutation
Ala 14	Cys 58	engineered mutation
Trp 20	Phe 64	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	217185.9
Non-Polymers*	Number of molecules	21
	Total formula weight	3414.4
All*	Total formula weight	220600.3

*Water molecules are not included.