6TIZ

DROSOPHILA GDP-TUBULIN Y222F MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	66.176, 126.732, 250.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.000 - 2.197
R-factor	0.1766
Rwork	0.175
R-free	0.20850
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3ryc
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BUSTER
Refinement software	BUSTER (2.10.3 (3-OCT-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.370	2.230
High resolution limit [Å]	2.197	2.197
Number of reflections	107685	29817
<I/σ(I)>	12.8	1.6
Completeness [%]	99.5
Redundancy	9.4
CC(1/2)	0.998	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG1500, LISO4, PIPES BUFFER, PH 6.8