6TEX

Crystal Structure of full-length Human Lysyl Hydroxylase LH3 - Val80Lys mutant - Cocrystal with Fe2+, Mn2+, UDP-Glucose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3	polymer	718	82814.5	1	UniProt (O60568) Pfam (PF25342) Pfam (PF25238) Pfam (PF03171)	Homo sapiens (Human)
2	B (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	C, D (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL) PubChem (753)
4	E (A)	2-OXOGLUTARIC ACID	non-polymer		146.1	1	Chemie (AKG) PubChem (51)
5	F (A)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	1	Chemie (UDP) PubChem (1158) PubChem (6031) PubChem (25323044) PubChem (25323048) PubChem (44298965) PubChem (53486216) PubChem (60098857) PubChem (57448939) PubChem (3084486) PubChem (91746160) PubChem (6101632) PubChem (92332560) PubChem (92332561) PubChem (92332562) PubChem (92332563) PubChem (93088131) PubChem (93630083) PubChem (6604175) PubChem (11811538) PubChem (12049241)
6	G, H (A)	FE (II) ION	non-polymer		55.8	2	Chemie (FE2) PubChem (27284)
7	I (A)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN) PubChem (27854)
8	J (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
9	K (A)	water	water		18.0	110	Chemie (HOH)

Sequence modifications

A: 25 - 738 (UniProt: O60568)

PDB	External Database	Details
Ser 24	-	expression tag
Lys 80	Val 80	engineered mutation
Ala 739	-	expression tag
Ala 740	-	expression tag
Ala 741	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	82814.5
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	8
	Total formula weight	1122.3
All*	Total formula weight	84361.1

*Water molecules are not included.