6T58

Structure determination of the transactivation domain of p53 in complex with S100A4 using annexin A2 as a crystallization chaperone

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cellular tumor antigen p53,Protein S100-A4,Protein S100-A4,Annexin A2	polymer	553	63182.7	2	UniProt (P04637) UniProt (P26447) UniProt (P07355) Pfam (PF08563) Pfam (PF18521) Pfam (PF01023) Pfam (PF00191)	Homo sapiens (Human)	Antigen NY-CO-13,Phosphoprotein p53,Tumor suppressor p53,Calvasculin,Metastasin,Placental calcium-binding protein,Protein Mts1,S100 calcium-binding protein A4,Calvasculin,Metastasin,Placental calcium-binding protein,Protein Mts1,S100 calcium-binding protein A4,Annexin II,Annexin-2,Calpactin I heavy chain,Calpactin-1 heavy chain,Chromobindin-8,Lipocortin II,Placental anticoagulant protein IV,PAP-IV,Protein I,p36
2	C, D, O, P (A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
3	E, F, G, H, I... (A, B)	CALCIUM ION	non-polymer		40.1	16	Chemie (CA)

Sequence modifications

A, B: 17 - 56 (UniProt: P04637)

PDB	External Database	Details
Gly 13	-	expression tag
Ser 14	-	expression tag
His 15	-	expression tag
Met 16	-	expression tag
Gly 57	-	linker
Gly 58	-	linker
Ser 59	-	linker
Gly 60	-	linker
His 61	-	linker

A, B: 62 - 154 (UniProt: P26447)
A, B: 161 - 252 (UniProt: P26447)

PDB	External Database	Details
Ser 155	-	linker
Ala 156	-	linker
Gly 157	-	linker
Ser 158	-	linker
Ala 159	-	linker
Gly 160	-	linker
Thr 253	-	linker
Ser 254	-	linker

A, B: 255 - 565 (UniProt: P07355)

PDB	External Database	Details
Glu 292	Ala 66	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	126365.5
Non-Polymers*	Number of molecules	20
	Total formula weight	1009.6
All*	Total formula weight	127375.1

*Water molecules are not included.