6T58
Structure determination of the transactivation domain of p53 in complex with S100A4 using annexin A2 as a crystallization chaperone
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001525 | biological_process | angiogenesis |
| A | 0001533 | cellular_component | cornified envelope |
| A | 0001765 | biological_process | membrane raft assembly |
| A | 0001786 | molecular_function | phosphatidylserine binding |
| A | 0001837 | biological_process | epithelial to mesenchymal transition |
| A | 0001921 | biological_process | positive regulation of receptor recycling |
| A | 0002020 | molecular_function | protease binding |
| A | 0002091 | biological_process | negative regulation of receptor internalization |
| A | 0003723 | molecular_function | RNA binding |
| A | 0003779 | molecular_function | actin binding |
| A | 0004859 | molecular_function | phospholipase inhibitor activity |
| A | 0004867 | molecular_function | serine-type endopeptidase inhibitor activity |
| A | 0005509 | molecular_function | calcium ion binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005544 | molecular_function | calcium-dependent phospholipid binding |
| A | 0005546 | molecular_function | phosphatidylinositol-4,5-bisphosphate binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005604 | cellular_component | basement membrane |
| A | 0005615 | cellular_component | extracellular space |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005765 | cellular_component | lysosomal membrane |
| A | 0005768 | cellular_component | endosome |
| A | 0005769 | cellular_component | early endosome |
| A | 0005811 | cellular_component | lipid droplet |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005912 | cellular_component | adherens junction |
| A | 0006900 | biological_process | vesicle budding from membrane |
| A | 0007155 | biological_process | cell adhesion |
| A | 0008092 | molecular_function | cytoskeletal protein binding |
| A | 0009986 | cellular_component | cell surface |
| A | 0010756 | biological_process | positive regulation of plasminogen activation |
| A | 0012506 | cellular_component | vesicle membrane |
| A | 0016020 | cellular_component | membrane |
| A | 0016323 | cellular_component | basolateral plasma membrane |
| A | 0016363 | cellular_component | nuclear matrix |
| A | 0019834 | molecular_function | phospholipase A2 inhibitor activity |
| A | 0030496 | cellular_component | midbody |
| A | 0031012 | cellular_component | extracellular matrix |
| A | 0031340 | biological_process | positive regulation of vesicle fusion |
| A | 0031902 | cellular_component | late endosome membrane |
| A | 0031982 | cellular_component | vesicle |
| A | 0032804 | biological_process | negative regulation of low-density lipoprotein particle receptor catabolic process |
| A | 0035578 | cellular_component | azurophil granule lumen |
| A | 0035749 | cellular_component | myelin sheath adaxonal region |
| A | 0036035 | biological_process | osteoclast development |
| A | 0042056 | molecular_function | chemoattractant activity |
| A | 0042383 | cellular_component | sarcolemma |
| A | 0042470 | cellular_component | melanosome |
| A | 0042789 | biological_process | mRNA transcription by RNA polymerase II |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0043123 | biological_process | positive regulation of canonical NF-kappaB signal transduction |
| A | 0043220 | cellular_component | Schmidt-Lanterman incisure |
| A | 0044090 | biological_process | positive regulation of vacuole organization |
| A | 0044548 | molecular_function | S100 protein binding |
| A | 0045121 | cellular_component | membrane raft |
| A | 0045921 | biological_process | positive regulation of exocytosis |
| A | 0045944 | biological_process | positive regulation of transcription by RNA polymerase II |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0046914 | molecular_function | transition metal ion binding |
| A | 0048306 | molecular_function | calcium-dependent protein binding |
| A | 0048471 | cellular_component | perinuclear region of cytoplasm |
| A | 0050767 | biological_process | regulation of neurogenesis |
| A | 0050786 | molecular_function | RAGE receptor binding |
| A | 0050918 | biological_process | positive chemotaxis |
| A | 0051051 | biological_process | negative regulation of transport |
| A | 0051240 | biological_process | positive regulation of multicellular organismal process |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0098609 | biological_process | cell-cell adhesion |
| A | 0098641 | molecular_function | cadherin binding involved in cell-cell adhesion |
| A | 1905581 | biological_process | positive regulation of low-density lipoprotein particle clearance |
| A | 1905602 | biological_process | positive regulation of receptor-mediated endocytosis involved in cholesterol transport |
| A | 1905686 | biological_process | positive regulation of plasma membrane repair |
| A | 1990665 | cellular_component | AnxA2-p11 complex |
| A | 1990667 | cellular_component | PCSK9-AnxA2 complex |
| B | 0001525 | biological_process | angiogenesis |
| B | 0001533 | cellular_component | cornified envelope |
| B | 0001765 | biological_process | membrane raft assembly |
| B | 0001786 | molecular_function | phosphatidylserine binding |
| B | 0001837 | biological_process | epithelial to mesenchymal transition |
| B | 0001921 | biological_process | positive regulation of receptor recycling |
| B | 0002020 | molecular_function | protease binding |
| B | 0002091 | biological_process | negative regulation of receptor internalization |
| B | 0003723 | molecular_function | RNA binding |
| B | 0003779 | molecular_function | actin binding |
| B | 0004859 | molecular_function | phospholipase inhibitor activity |
| B | 0004867 | molecular_function | serine-type endopeptidase inhibitor activity |
| B | 0005509 | molecular_function | calcium ion binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005544 | molecular_function | calcium-dependent phospholipid binding |
| B | 0005546 | molecular_function | phosphatidylinositol-4,5-bisphosphate binding |
| B | 0005576 | cellular_component | extracellular region |
| B | 0005604 | cellular_component | basement membrane |
| B | 0005615 | cellular_component | extracellular space |
| B | 0005634 | cellular_component | nucleus |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005765 | cellular_component | lysosomal membrane |
| B | 0005768 | cellular_component | endosome |
| B | 0005769 | cellular_component | early endosome |
| B | 0005811 | cellular_component | lipid droplet |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0005912 | cellular_component | adherens junction |
| B | 0006900 | biological_process | vesicle budding from membrane |
| B | 0007155 | biological_process | cell adhesion |
| B | 0008092 | molecular_function | cytoskeletal protein binding |
| B | 0009986 | cellular_component | cell surface |
| B | 0010756 | biological_process | positive regulation of plasminogen activation |
| B | 0012506 | cellular_component | vesicle membrane |
| B | 0016020 | cellular_component | membrane |
| B | 0016323 | cellular_component | basolateral plasma membrane |
| B | 0016363 | cellular_component | nuclear matrix |
| B | 0019834 | molecular_function | phospholipase A2 inhibitor activity |
| B | 0030496 | cellular_component | midbody |
| B | 0031012 | cellular_component | extracellular matrix |
| B | 0031340 | biological_process | positive regulation of vesicle fusion |
| B | 0031902 | cellular_component | late endosome membrane |
| B | 0031982 | cellular_component | vesicle |
| B | 0032804 | biological_process | negative regulation of low-density lipoprotein particle receptor catabolic process |
| B | 0035578 | cellular_component | azurophil granule lumen |
| B | 0035749 | cellular_component | myelin sheath adaxonal region |
| B | 0036035 | biological_process | osteoclast development |
| B | 0042056 | molecular_function | chemoattractant activity |
| B | 0042383 | cellular_component | sarcolemma |
| B | 0042470 | cellular_component | melanosome |
| B | 0042789 | biological_process | mRNA transcription by RNA polymerase II |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0043123 | biological_process | positive regulation of canonical NF-kappaB signal transduction |
| B | 0043220 | cellular_component | Schmidt-Lanterman incisure |
| B | 0044090 | biological_process | positive regulation of vacuole organization |
| B | 0044548 | molecular_function | S100 protein binding |
| B | 0045121 | cellular_component | membrane raft |
| B | 0045921 | biological_process | positive regulation of exocytosis |
| B | 0045944 | biological_process | positive regulation of transcription by RNA polymerase II |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0046914 | molecular_function | transition metal ion binding |
| B | 0048306 | molecular_function | calcium-dependent protein binding |
| B | 0048471 | cellular_component | perinuclear region of cytoplasm |
| B | 0050767 | biological_process | regulation of neurogenesis |
| B | 0050786 | molecular_function | RAGE receptor binding |
| B | 0050918 | biological_process | positive chemotaxis |
| B | 0051051 | biological_process | negative regulation of transport |
| B | 0051240 | biological_process | positive regulation of multicellular organismal process |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 0098609 | biological_process | cell-cell adhesion |
| B | 0098641 | molecular_function | cadherin binding involved in cell-cell adhesion |
| B | 1905581 | biological_process | positive regulation of low-density lipoprotein particle clearance |
| B | 1905602 | biological_process | positive regulation of receptor-mediated endocytosis involved in cholesterol transport |
| B | 1905686 | biological_process | positive regulation of plasma membrane repair |
| B | 1990665 | cellular_component | AnxA2-p11 complex |
| B | 1990667 | cellular_component | PCSK9-AnxA2 complex |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 601 |
| Chain | Residue |
| A | CYS359 |
| A | ARG361 |
| A | ARG405 |
| A | LEU485 |
| A | LYS492 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 602 |
| Chain | Residue |
| A | VAL524 |
| A | PRO332 |
| A | ASP336 |
| A | GLU365 |
| A | SER520 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | binding site for residue CA A 603 |
| Chain | Residue |
| A | ASP124 |
| A | ASN126 |
| A | ASP128 |
| A | GLU130 |
| A | GLU135 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue CA A 604 |
| Chain | Residue |
| A | GLY428 |
| A | ARG431 |
| A | GLY433 |
| A | GLU473 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | binding site for residue CA A 605 |
| Chain | Residue |
| A | GLY276 |
| A | VAL277 |
| A | GLU279 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | binding site for residue CA A 606 |
| Chain | Residue |
| A | MET344 |
| A | GLY346 |
| A | LEU347 |
| A | GLY348 |
| A | ASP388 |
| site_id | AC7 |
| Number of Residues | 7 |
| Details | binding site for residue CA A 607 |
| Chain | Residue |
| A | ASP222 |
| A | SER223 |
| A | ASN224 |
| A | ARG225 |
| A | ASP226 |
| A | GLU228 |
| A | GLU233 |
| site_id | AC8 |
| Number of Residues | 2 |
| Details | binding site for residue CA A 608 |
| Chain | Residue |
| A | THR349 |
| A | GLU351 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | binding site for residue CA A 609 |
| Chain | Residue |
| A | SER179 |
| A | GLU182 |
| A | GLY183 |
| A | ASP184 |
| A | LYS187 |
| A | ASN189 |
| A | GLU192 |
| site_id | AD1 |
| Number of Residues | 4 |
| Details | binding site for residue CA A 610 |
| Chain | Residue |
| A | MET504 |
| A | GLY506 |
| A | GLY508 |
| A | ASP548 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue CA A 611 |
| Chain | Residue |
| A | LYS314 |
| A | LEU317 |
| A | GLU322 |
| B | ASP296 |
| site_id | AD3 |
| Number of Residues | 5 |
| Details | binding site for residue CA A 612 |
| Chain | Residue |
| A | SER81 |
| A | GLU84 |
| A | ASP86 |
| A | LYS89 |
| A | GLU94 |
| site_id | AD4 |
| Number of Residues | 7 |
| Details | binding site for residue GOL B 601 |
| Chain | Residue |
| B | PRO332 |
| B | ASP336 |
| B | ARG361 |
| B | GLU365 |
| B | SER520 |
| B | ARG521 |
| B | VAL524 |
| site_id | AD5 |
| Number of Residues | 5 |
| Details | binding site for residue GOL B 602 |
| Chain | Residue |
| B | CYS359 |
| B | ARG361 |
| B | ASN363 |
| B | ARG405 |
| B | LYS492 |
| site_id | AD6 |
| Number of Residues | 5 |
| Details | binding site for residue CA B 603 |
| Chain | Residue |
| B | MET504 |
| B | GLY506 |
| B | GLY508 |
| B | THR509 |
| B | ASP548 |
| site_id | AD7 |
| Number of Residues | 4 |
| Details | binding site for residue CA B 604 |
| Chain | Residue |
| B | MET344 |
| B | GLY346 |
| B | GLY348 |
| B | ASP388 |
| site_id | AD8 |
| Number of Residues | 1 |
| Details | binding site for residue CA B 605 |
| Chain | Residue |
| B | SER460 |
| site_id | AD9 |
| Number of Residues | 5 |
| Details | binding site for residue CA B 606 |
| Chain | Residue |
| B | GLY428 |
| B | VAL429 |
| B | ARG431 |
| B | GLY433 |
| B | GLU473 |
| site_id | AE1 |
| Number of Residues | 3 |
| Details | binding site for residue CA B 607 |
| Chain | Residue |
| B | GLY276 |
| B | VAL277 |
| B | GLU279 |
| site_id | AE2 |
| Number of Residues | 3 |
| Details | binding site for residue CA B 608 |
| Chain | Residue |
| B | LYS314 |
| B | LEU317 |
| B | GLU322 |
Functional Information from PROSITE/UniProt
| site_id | PS00018 |
| Number of Residues | 13 |
| Details | EF_HAND_1 EF-hand calcium-binding domain. DSNRDNEVDfqEY |
| Chain | Residue | Details |
| A | ASP124-TYR136 | |
| A | ASP222-TYR234 |
| site_id | PS00223 |
| Number of Residues | 53 |
| Details | ANNEXIN_1 Annexin repeat signature. GVdevtivniLtnRsneQrqDiafaYqrrtkkeLasaLksalsGhletvIlgL |
| Chain | Residue | Details |
| A | GLY276-LEU328 | |
| A | GLY348-LEU400 | |
| A | GLY433-LEU485 | |
| A | GLY508-LEU560 |
| site_id | PS00303 |
| Number of Residues | 22 |
| Details | S100_CABP S-100/ICaBP type calcium binding protein signature. LMsnLDsnrDnevDFqEYcvFL |
| Chain | Residue | Details |
| A | LEU119-LEU140 | |
| A | LEU217-LEU238 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | Motif: {"description":"TADI"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphothreonine; by CK1, VRK1 and VRK2","evidences":[{"source":"PubMed","id":"10606744","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"10951572","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16704422","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"31527692","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine; by CHEK2, CK1 and PLK3","evidences":[{"source":"PubMed","id":"10570149","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11447225","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11551930","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12810724","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20041275","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine; by MAPKAPK5","evidences":[{"source":"PubMed","id":"17254968","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine; by CDK5, DYRK2, HIPK2 and PKC/PRKCG","evidences":[{"source":"PubMed","id":"11740489","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11780126","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16377624","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17349958","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17591690","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"21597459","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 142 |
| Details | Repeat: {"description":"Annexin 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU01245","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 142 |
| Details | Repeat: {"description":"Annexin 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU01245","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 144 |
| Details | Repeat: {"description":"Annexin 3","evidences":[{"source":"PROSITE-ProRule","id":"PRU01245","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 142 |
| Details | Repeat: {"description":"Annexin 4","evidences":[{"source":"PROSITE-ProRule","id":"PRU01245","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine; by PKC","evidences":[{"source":"PubMed","id":"28669632","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"2946940","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P07356","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P07356","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI15 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI16 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P07356","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI17 |
| Number of Residues | 35 |
| Details | Domain: {"description":"EF-hand 1","evidences":[{"evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI18 |
| Number of Residues | 35 |
| Details | Domain: {"description":"EF-hand 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI19 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI20 |
| Number of Residues | 5 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI21 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
