6T30
Streptavidin variants harbouring an artificial organocatalyst based cofactor
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | B, A | Streptavidin | polymer | 159 | 16454.9 | 2 | UniProt (P22629) Pfam (PF01382) In PDB | Streptomyces avidinii | |
2 | B, A | 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-(1-pyridin-4-ylpiperidin-4-yl)pentanamide | non-polymer | 403.5 | 2 | Chemie (HL9) | |||
3 | B, A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
4 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
5 | water | water | 18.0 | 114 | Chemie (HOH) |
Sequence modifications
B, A: 14 - 159 (UniProt: P22629)
PDB | External Database | Details |
---|---|---|
Met 1 | - | initiating methionine |
Ala 2 | - | expression tag |
Ser 3 | - | expression tag |
Met 4 | - | expression tag |
Thr 5 | - | expression tag |
Gly 6 | - | expression tag |
Gly 7 | - | expression tag |
Gln 8 | - | expression tag |
Gln 9 | - | expression tag |
Met 10 | - | expression tag |
Gly 11 | - | expression tag |
Arg 12 | - | expression tag |
Asp 13 | - | expression tag |
Ala 47 | Val 71 | engineered mutation |
Lys 49 | Asn 73 | engineered mutation |
Ala 114 | Thr 138 | engineered mutation |
Gly 119 | Ala 143 | engineered mutation |
Ala 121 | Lys 145 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 32909.8 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 1085.4 | |
All* | Total formula weight | 33995.2 |