6T30
Streptavidin variants harbouring an artificial organocatalyst based cofactor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 55.132, 55.132, 131.209 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.750 - 1.800 |
| R-factor | 0.1885 |
| Rwork | 0.186 |
| R-free | 0.22970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6t1e |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.274 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.750 | 1.865 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 21936 | 2112 |
| <I/σ(I)> | 17.36 | |
| Completeness [%] | 98.9 | |
| Redundancy | 10.9 | |
| CC(1/2) | 0.999 | 0.366 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M di-Sodium hydrogen phosphate 20% PEG 3350 |
