6T15

The III2-IV(5B)1 respiratory supercomplex from S. cerevisiae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, K (A, L)	CYTOCHROME B-C1 COMPLEX SUBUNIT 1, MITOCHONDRIAL; SYNONYM: COMPLEX III SUBUNIT 1, CORE PROTEIN I, UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX CORE PROTEIN 1	polymer	431	47459.3	2	UniProt (P07256) Pfam (PF00675) Pfam (PF05193)	Saccharomyces cerevisiae S288C
2	B, L (B, M)	CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL; SYNONYM: COMPLEX III SUBUNIT 2, CORE PROTEIN II, UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX CORE PROTEIN 2	polymer	352	38751.9	2	UniProt (P07257) Pfam (PF00675)	Saccharomyces cerevisiae S288C
3	C, M (C, N)	CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL; SYNONYM: COMPLEX III SUBUNIT 2, CORE PROTEIN II, UBIQUINOL-CYTOCHROME-C COMPLEX CORE PROTEIN 2	polymer	385	43686.6	2	UniProt (P00163) Pfam (PF00033) Pfam (PF00032)	Saccharomyces cerevisiae S288C
4	D, N (D, O)	CYTOCHROME C1, HEME PROTEIN, MITOCHONDRIAL; SYNONYM: COMPLEX III SUBUNIT 4, COMPLEX III SUBUNIT IV, CYTOCHROME B-C1 COMPLEX SUBUNIT 4, UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX CYTOCHROME C1 SUBUNIT, CYTOCHROME C-1	polymer	248	27807.4	2	UniProt (P07143) Pfam (PF02167)	Saccharomyces cerevisiae S288C
5	E, O (E, P)	CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL; SYNONYM: COMPLEX III SUBUNIT 5, RIESKE IRON-SULFUR PROTEIN, RISP, UBIQUINOL-CYTOCHROME C REDUCTASE IRON-SULFUR SUBUNIT	polymer	185	20123.0	2	UniProt (P08067) Pfam (PF02921) Pfam (PF00355)	Saccharomyces cerevisiae S288C
6	F, P (F, Q)	CYTOCHROME B-C1 COMPLEX SUBUNIT 6; SYNONYM: COMPLEX III SUBUNIT 6,COMPLEX III SUBUNIT VI,CYTOCHROME C1 NON-HEME 17 KDA PROTEIN,MITOCHONDRIAL HINGE PROTEIN,UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 17 KDA PROTEIN	polymer	147	17276.1	2	UniProt (P00127) Pfam (PF02320)	Saccharomyces cerevisiae S288C
7	G, Q (G, R)	CYTOCHROME B-C1 COMPLEX SUBUNIT 7; SYNONYM: COMPLEX III SUBUNIT 7,COMPLEX III SUBUNIT VII,UBIQUINOL-CYTOCHROME C REDUCTASE C REDUCTASE COMPLEX 14 KDA PROTEIN	polymer	127	14583.8	2	UniProt (P00128) Pfam (PF02271)	Saccharomyces cerevisiae S288C
8	H, R (H, S)	CYTOCHROME B-C1 COMPLEX SUBUNIT 8; SYNONYM: COMPLEX III SUBUNIT 8,COMPLEX III SUBUNIT VII,UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 11 KDA PROTEIN,UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX UBIQUINONE-BINDING PROTEIN QP-C	polymer	94	10987.5	2	UniProt (P08525) Pfam (PF02939)	Saccharomyces cerevisiae S288C
9	I, S (I, T)	CYTOCHROME B-C1 COMPLEX SUBUNIT 9; SYNONYM: COMPLEX III SUBUNIT 9, COMPLEX III SUBUNIT X, CYTOCHROME C1 NON-HEME 7.3 KDA PROTEIN, UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 7.3 KDA PROTEIN	polymer	66	7485.3	2	UniProt (P22289) Pfam (PF05365)	Saccharomyces cerevisiae S288C
10	J, T (J, U)	CYTOCHROME B-C1 COMPLEX SUBUNIT 10; SYNONYM: COMPLEX III SUBUNIT 10,COMPLEX III SUBUNIT XI, UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 8.5 KDA PROTEIN	polymer	77	8602.9	2	UniProt (P37299) Pfam (PF09796)	Saccharomyces cerevisiae S288C
11	U (a)	CYTOCHROME C OXIDASE SUBUNIT 1; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE I, COX1	polymer	534	58832.6	1	UniProt (P00401) Pfam (PF00115)	Saccharomyces cerevisiae S288C
12	V (b)	CYTOCHROME C OXIDASE SUBUNIT 2; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE II, COX2	polymer	236	26779.8	1	UniProt (P00410) Pfam (PF02790) Pfam (PF00116)	Saccharomyces cerevisiae S288C
13	W (c)	CYTOCHROME C OXIDASE SUBUNIT 3; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE III, COX3	polymer	269	30383.6	1	UniProt (P00420) Pfam (PF00510)	Saccharomyces cerevisiae S288C
14	X (d)	CYTOCHROME C OXIDASE SUBUNIT 4, MITOCHONDRIAL; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE IV, COX4	polymer	130	14188.9	1	UniProt (P04037) Pfam (PF01215)	Saccharomyces cerevisiae S288C
15	Y (e)	CYTOCHROME C OXIDASE POLYPEPTIDE 5B, MITOCHONDRIAL; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE VB, COX5B	polymer	134	15325.4	1	UniProt (P00425) Pfam (PF02936)	Saccharomyces cerevisiae S288C
16	Z (f)	CYTOCHROME C OXIDASE SUBUNIT 6, MITOCHONDRIAL; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE VI, COX6	polymer	108	12642.0	1	UniProt (P00427) Pfam (PF02284)	Saccharomyces cerevisiae S288C
17	AA (g)	CYTOCHROME C OXIDASE SUBUNIT 7; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE VII, COX7	polymer	59	6811.2	1	UniProt (P10174) Pfam (PF02238)	Saccharomyces cerevisiae S288C
18	BA (h)	Cytochrome c oxidase polypeptide VIII, mitochondrial	polymer	51	5737.7	1	UniProt (P04039) Pfam (PF02935)	Saccharomyces cerevisiae S288c (Baker's yeast)
19	CA (i)	CYTOCHROME C OXIDASE SUBUNIT 7A; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE VIIA, COX9	polymer	55	6471.7	1	UniProt (P07255)	Saccharomyces cerevisiae S288C
20	DA (j)	Cytochrome c oxidase subunit 6B	polymer	82	9668.7	1	UniProt (Q01519) Pfam (PF02297)	Saccharomyces cerevisiae S288c (Baker's yeast)	Cytochrome c oxidase polypeptide VIb
21	EA (k)	CYTOCHROME C OXIDASE SUBUNIT 6A, MITOCHONDRIAL; CYTOCHROME C OXIDASE POLYPEPTIDE VIA, COX13	polymer	131	15117.0	1	UniProt (P32799) Pfam (PF02046)	Saccharomyces cerevisiae S288C
22	FA (l)	COX26; SYNONYM: Uncharacterized protein YDR119W-A	polymer	66	7461.7	1	UniProt (Q2V2P9)	Saccharomyces cerevisiae S288C
23	GA (m)	RCF2; SYNONYM: Respiratory supercomplex factor 2	polymer	224	25381.1	1	UniProt (P53721) Pfam (PF04588)	Saccharomyces cerevisiae S288C
24	BB, CB, HA, HB, JB... (N, A, P, S, C...)	DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE	non-polymer		692.0	18	Chemie (PEF)
25	IA, JA, YA, ZA (C, N)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	4	Chemie (HEM)
26	AB, FB, IB, KA, PA... (N, O, S, C, D...)	CARDIOLIPIN	non-polymer		1464.0	7	Chemie (CDL)
27	DB, KB, LB, NA, UA... (N, S, T, C, I...)	1,2-DIACYL-SN-GLYCERO-3-PHOSHOCHOLINE	non-polymer		734.0	6	Chemie (PCF)
28	EB, OA (O, D)	HEME C	non-polymer		618.5	2	Chemie (HEC)
29	GB, QA (P, E)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
30	NB (a)	COPPER (II) ION	non-polymer		63.5	1	Chemie (CU)
31	OB, PB (a)	HEME-A	non-polymer		852.8	2	Chemie (HEA)
32	QB (a)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
33	SB (b)	DINUCLEAR COPPER ION	non-polymer		127.1	1	Chemie (CUA)
34	XB (d)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

k: 10 - 129 (UniProt: P32799)

PDB	External Database	Details
Gly 130	-	expression tag
Ala 131	-	expression tag
Arg 132	-	expression tag
Gly 133	-	expression tag
Ser 134	-	expression tag
His 135	-	expression tag
His 136	-	expression tag
His 137	-	expression tag
His 138	-	expression tag
His 139	-	expression tag
His 140	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	33
	Total formula weight	708328.9
Non-Polymers*	Number of molecules	45
	Total formula weight	33148.4
All*	Total formula weight	741477.4

*Water molecules are not included.