6SZO

The glucuronoyl esterase OtCE15A S267A variant from Opitutus terrae in complex with D-galacturonate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	glucuronoyl esterase OtCE15A	polymer	421	46132.5	1	UniProt (B1ZMF4) Pfam (PF22244)	Opitutus terrae PB90-1
2	B (A)	beta-D-galactopyranuronic acid	non-polymer		194.1	1	Chemie (GTR)
3	C, D, E, F (A)	DIMETHYL SULFOXIDE	non-polymer		78.1	4	Chemie (DMS)
4	G (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	AA, BA, CA, DA, EA... (A)	1,2-ETHANEDIOL	non-polymer		62.1	24	Chemie (EDO)
6	FA, GA, HA, IA (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	4	Chemie (PEG)
7	JA, KA, LA, MA (A)	TRIETHYLENE GLYCOL	non-polymer		150.2	4	Chemie (PGE)
8	NA (A)	water	water		18.0	122	Chemie (HOH)

Sequence modifications

A: 33 - 432 (UniProt: B1ZMF4)

PDB	External Database	Details
Met 12	-	initiating methionine
Gly 13	-	expression tag
Ser 14	-	expression tag
Ser 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
Ser 22	-	expression tag
Ser 23	-	expression tag
Glu 24	-	expression tag
Asn 25	-	expression tag
Leu 26	-	expression tag
Tyr 27	-	expression tag
Phe 28	-	expression tag
Gln 29	-	expression tag
Gly 30	-	expression tag
His 31	-	expression tag
Ser 32	-	expression tag
Ala 267	Ser 267	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46132.5
Non-Polymers*	Number of molecules	38
	Total formula weight	3045.8
All*	Total formula weight	49178.3

*Water molecules are not included.