6SQ2

Structure of a phosphomimetic switch 2 variant of Rab8a in complex with the phospho-Rab binding domain of RILPL2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ras-related protein Rab-8A	polymer	184	21077.2	2	UniProt (P61006) Pfam (PF00071)	Homo sapiens (Human)	Oncogene c-mel
2	C, D (D, E)	RILP-like protein 2	polymer	43	5296.0	2	UniProt (Q969X0) Pfam (PF11461)	Homo sapiens (Human)	Rab-interacting lysosomal protein-like 2,p40phox-binding protein
3	E, G (A, B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
4	F, H (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	I, J, K, L (A, B, D, E)	water	water		18.0	513	Chemie (HOH)

Sequence modifications

A, B: 1 - 181 (UniProt: P61006)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Leu 67	Gln 67	engineered mutation
Glu 72	Thr 72	engineered mutation

D, E: 129 - 165 (UniProt: Q969X0)

PDB	External Database	Details
His 123	-	expression tag
His 124	-	expression tag
His 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52746.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1095.0
All*	Total formula weight	53841.4

*Water molecules are not included.