6SPQ
Structure of the Escherichia coli methionyl-tRNA synthetase variant VI298 complexed with methionine
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Methionine--tRNA ligase | polymer | 568 | 64744.0 | 1 | UniProt (A0A0F3U9S7) Pfam (PF09334) Pfam (PF19303) UniProt (by SIFTS) (P00959) In PDB | Escherichia coli | Methionyl-tRNA synthetase,MetRS |
2 | A | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
3 | A | METHIONINE | non-polymer | 149.2 | 1 | Chemie (MET) | |||
4 | A | CITRIC ACID | non-polymer | 192.1 | 2 | Chemie (CIT) | |||
5 | A | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
6 | water | water | 18.0 | 685 | Chemie (HOH) |
Sequence modifications
A: 1 - 547 (UniProt: A0A0F3U9S7)
PDB | External Database | Details |
---|---|---|
Met -20 | - | initiating methionine |
Gly -19 | - | expression tag |
Ser -18 | - | expression tag |
Ser -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Ser -10 | - | expression tag |
Ser -9 | - | expression tag |
Gly -8 | - | expression tag |
Leu -7 | - | expression tag |
Val -6 | - | expression tag |
Pro -5 | - | expression tag |
Arg -4 | - | expression tag |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
His -1 | - | expression tag |
Ser 0 | - | expression tag |
Ile 298 | Val 299 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 64744.0 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 967.2 | |
All* | Total formula weight | 65711.3 |