6SPQ

Structure of the Escherichia coli methionyl-tRNA synthetase variant VI298 complexed with methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	78.420, 45.130, 86.150
Unit cell angles	90.00, 107.49, 90.00

Refinement procedure

Resolution	48.530 - 1.380
R-factor	0.1287
Rwork	0.127
R-free	0.16180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.530	1.470
High resolution limit [Å]	1.380	1.380
Number of reflections	116836	18108
<I/σ(I)>	15.9	1.4
Completeness [%]	99.1
Redundancy	6.8
CC(1/2)	0.999	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	1.08 M ammonium citrate, 10 mM potassium phosphate, pH 7.0