6SNI
Cryo-EM structure of nanodisc reconstituted yeast ALG6 in complex with 6AG9 Fab
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (X) | Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase | polymer | 562 | 64423.2 | 1 | UniProt (Q12001) Pfam (PF03155) | Saccharomyces cerevisiae (Baker's yeast) | Asparagine-linked glycosylation protein 6,Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase,Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase |
| 2 | B (H) | 6AG9-Fab heavy chain | polymer | 234 | 24953.7 | 1 | synthetic construct | ||
| 3 | C (L) | 6AG9-Fab light chain | polymer | 217 | 23650.3 | 1 | synthetic construct | ||
| 4 | D, E, F, G, H (X) | PHOSPHATIDYLETHANOLAMINE | non-polymer | 734.0 | 5 | Chemie (PTY) | |||
| 5 | I, J, K (X, H) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 3 | Chemie (Y01) | |||
| 6 | L, M (X, H) | water | water | 18.0 | 7 | Chemie (HOH) |
Sequence modifications
X: 1 - 544 (UniProt: Q12001)
| PDB | External Database | Details |
|---|---|---|
| Gly -17 | - | expression tag |
| Pro -16 | - | expression tag |
| Gly -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Ser -13 | - | expression tag |
| Thr -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Arg -10 | - | expression tag |
| Leu -9 | - | expression tag |
| Ala -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Ala -6 | - | expression tag |
| Gly -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Glu -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Val -1 | - | expression tag |
| Asp 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 113027.1 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 5130.4 | |
| All* | Total formula weight | 118157.5 |
