6SMN

A. thaliana serine hydroxymethyltransferase isoform 2 (AtSHMT2) in complex with methotrexate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Serine hydroxymethyltransferase 2, mitochondrial	polymer	480	53545.0	3	UniProt (Q94C74) Pfam (PF00464) In PDB	Arabidopsis thaliana (Mouse-ear cress)	AtSHMT2,Glycine hydroxymethyltransferase 2,Serine methylase 2
2	D	Serine hydroxymethyltransferase 2, mitochondrial	polymer	480	53773.2	1	UniProt (Q94C74) Pfam (PF00464) In PDB	Arabidopsis thaliana (Mouse-ear cress)	AtSHMT2,Glycine hydroxymethyltransferase 2,Serine methylase 2
3	A, C, B	[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE	non-polymer		336.2	3	Chemie (PLS)
4	B, D, A, C	1,2-ETHANEDIOL	non-polymer		62.1	48	Chemie (EDO)
5	A, C, B	METHOTREXATE	non-polymer		454.4	4	Chemie (MTX)
6	D	SERINE	non-polymer		105.1	1	Chemie (SER)
7		water	water		18.0	1922	Chemie (HOH)

Sequence modifications

A, B, C: 41 - 517 (UniProt: Q94C74)

PDB	External Database	Details
Ser 38	-	expression tag
Asn 39	-	expression tag
Ala 40	-	expression tag

D: 41 - 517 (UniProt: Q94C74)

PDB	External Database	Details
Ser 38	-	expression tag
Asn 39	-	expression tag
Ala 40	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	214408.3
Non-Polymers*	Number of molecules	56
	Total formula weight	5910.8
All*	Total formula weight	220319.1

*Water molecules are not included.