6SK2

HsNMT1 in complex with both MyrCoA and Acetylated-GKSFSKPR peptide reveals N-terminal Lysine Myristoylation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Glycylpeptide N-tetradecanoyltransferase 1	polymer	402	46465.4	2	UniProt (P30419) Pfam (PF01233) Pfam (PF02799) In PDB	Homo sapiens (Human)	Myristoyl-CoA:protein N-myristoyltransferase 1,Type I N-myristoyltransferase,Peptide N-myristoyltransferase 1
2	D, F	Apoptosis-inducing factor 3	polymer	9	935.1	2	UniProt (Q96NN9) In PDB	Homo sapiens	Apoptosis-inducing factor-like protein
3	A, B	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4	A	COENZYME A	non-polymer		767.5	1	Chemie (COA)
5	B	TETRADECANOYL-COA	non-polymer		977.9	1	Chemie (MYA)
6	D	MYRISTIC ACID	non-polymer		228.4	1	Chemie (MYR)
7		water	water		18.0	727	Chemie (HOH)

Sequence modifications

A, B: 99 - 496 (UniProt: P30419)

PDB	External Database	Details
Gly 95	-	expression tag
Gly 96	-	expression tag
Ser 97	-	expression tag
Glu 98	-	expression tag

D, F: 5 - 8 (UniProt: Q96NN9)

PDB	External Database	Details
Ace 101	-	acetylation
Gly 2	-	engineered mutation
Lys 3	-	engineered mutation
Ser 4	-	engineered mutation
Arg 9	-	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	94801.0
Non-Polymers*	Number of molecules	6
	Total formula weight	2250.1
All*	Total formula weight	97051.1

*Water molecules are not included.