6SK2
HsNMT1 in complex with both MyrCoA and Acetylated-GKSFSKPR peptide reveals N-terminal Lysine Myristoylation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.984002 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 78.818, 178.442, 58.271 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.788 - 1.900 |
R-factor | 0.174280163469 |
Rwork | 0.172 |
R-free | 0.21999 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5o9t |
RMSD bond length | 0.006 |
RMSD bond angle | 0.858 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.790 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.102 | 0.941 |
Rmeas | 0.106 | 0.975 |
Rpim | 0.028 | 0.252 |
Number of reflections | 131286 | 4177 |
<I/σ(I)> | 15.4 | |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 14.2 | 14.6 |
CC(1/2) | 0.999 | 0.934 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 22% PEG8K, 100mM Sodium Citrate pH 5.6, 100mM MgCl2, 100mM NaCl |