6SJA

Structure of HPV16 E6 oncoprotein in complex with IRF3 LxxLL motif

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Maltose/maltodextrin-binding periplasmic protein,Interferon regulatory factor 3	polymer	383	41733.2	1	UniProt (P0AEX9) UniProt (Q14653) Pfam (PF01547) In PDB	Escherichia coli	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,IRF-3
2	B	Protein E6	polymer	153	18439.3	1	UniProt (P03126) Pfam (PF00518) In PDB	Human papillomavirus type 16
3	C	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		828.7	1	In PDB GlyTouCan (G50146AM)		alpha-maltopentaose
4	B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5		water	water		18.0	481	Chemie (HOH)

Sequence modifications

A: 2 - 362 (UniProt: P0AEX9)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 83	Asp 108	conflict
Ala 84	Lys 109	engineered mutation
Ala 240	Lys 265	engineered mutation
Ala 360	Glu 385	engineered mutation
Ala 363	-	linker
Ala 364	-	linker
Ala 365	-	linker
Gln 366	-	linker
Thr 367	-	linker
Asn 368	-	linker
Ala 369	-	linker
Ala 370	-	linker
Ala 371	-	linker

A: 2137 - 2148 (UniProt: Q14653)
B: 1000 - 1151 (UniProt: P03126)

PDB	External Database	Details
Gly 999	-	expression tag
Arg 1047	Phe 54	engineered mutation
Ser 1080	Cys 87	engineered mutation
Ser 1097	Cys 104	engineered mutation
Ser 1111	Cys 118	engineered mutation
Ser 1140	Cys 147	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60172.5
Branched	Number of molecules	1
	Total formula weight	828.7
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	61132.0

*Water molecules are not included.