6SHB

Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 1, in the presence of ssDNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	CRISPR-associated protein, Cmr5 family	polymer	155	17961.8	3	UniProt (F0NDX5)	Sulfolobus islandicus REY15A
2	D, E, F, G (D, E, F, G)	CRISPR-associated RAMP protein, Cmr4 family	polymer	286	32322.4	4	UniProt (F0NDX6) Pfam (PF03787)	Sulfolobus islandicus REY15A
3	H (I)	CRISPR-associated RAMP protein, Cmr6 family	polymer	296	33945.9	1	UniProt (F0NDX3) Pfam (PF03787)	Sulfolobus islandicus REY15A
4	I (J)	Cmr1,CRISPR-associated RAMP protein, Cmr1 family	polymer	476	55389.0	1	UniProt (F0NDX4) Pfam (PF03787)	Sulfolobus islandicus REY15A
5	J (U)	Cognate target RNA (43-MER)	polymer	46	14704.7	1		synthetic construct
6	K (V)	crRNA (49-MER)	polymer	51	16409.9	1	GenBank (323473489)	Sulfolobus islandicus REY15A
7	AA, BA, CA, DA, EA... (L, M, N, O, P...)	CRISPR-associated protein Cmrx	polymer	174	19705.6	26	UniProt (F0NDX7) Pfam (PF19021)	Sulfolobus islandicus REY15A
8	HA (K)	CRISPR-associated protein, Cmr2 family	polymer	1037	120856.5	1	UniProt (F0NDX2) Pfam (PF12469) Pfam (PF22335)	Sulfolobus islandicus REY15A
9	KA (H)	CRISPR-associated protein, Cmr3 family	polymer	313	36008.5	1	UniProt (F0NDX1) Pfam (PF09700)	Sulfolobus islandicus REY15A
10	NA (K)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
11	OA, PA (K)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
12	QA, RA, SA (K)	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)

Sequence modifications

D, E, F, G: 1 - 286 (UniProt: F0NDX6)

PDB	External Database	Details
Ala 31	Asp 31	engineered mutation

I: 1 - 283 (UniProt: F0NDX3)

PDB	External Database	Details
Ala 284	-	expression tag
Ala 285	-	expression tag
Ala 286	-	expression tag
His 287	-	expression tag
His 288	-	expression tag
His 289	-	expression tag
His 290	-	expression tag
His 291	-	expression tag
His 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag

J: 1 - 5 (PDB: 6SHB)
J: 6 - 476 (UniProt: F0NDX4)
V: 1 - 51 (GenBank: 323473489)

PDB	External Database	Details
A 1	C 2068518	conflict
U 3	G 2068520	conflict

Contents of the asymmetric unit

Polymers	Number of chains	39
	Total formula weight	972835.2
Non-Polymers*	Number of molecules	6
	Total formula weight	1242.6
All*	Total formula weight	974077.8

*Water molecules are not included.