6SB0

cryo-EM structure of mTORC1 bound to PRAS40-fused active RagA/C GTPases

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	mTOR,Serine/threonine-protein kinase mTOR,mTOR,Serine/threonine-protein kinase mTOR	polymer	2549	287012.0	2	UniProt (P42345) Pfam (PF11865) Pfam (PF02259) Pfam (PF08771) Pfam (PF00454) Pfam (PF02260) In PDB	Homo sapiens (Human)	FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP12-rapamycin complex-associated protein,Mammalian target of rapamycin,mTOR,Mechanistic target of rapamycin,Rapamycin and FKBP12 target 1,Rapamycin target protein 1,FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP12-rapamycin complex-associated protein,Mammalian target of rapamycin,mTOR,Mechanistic target of rapamycin,Rapamycin and FKBP12 target 1,Rapamycin target protein 1
2	E, H	Target of rapamycin complex subunit LST8	polymer	326	35910.1	2	UniProt (Q9BVC4) Pfam (PF00400) In PDB	Homo sapiens (Human)	TORC subunit LST8,G protein beta subunit-like,Protein GbetaL,Mammalian lethal with SEC13 protein 8,mLST8
3	C, I	Ras-related GTP-binding protein A	polymer	313	36600.2	2	UniProt (Q7L523) Pfam (PF04670) In PDB	Homo sapiens (Human)	RagA,Adenovirus E3 14.7 kDa-interacting protein 1,FIP-1
4	D, J	Ras-related GTP-binding protein C	polymer	399	44284.8	2	UniProt (Q9HB90) Pfam (PF04670) In PDB	Homo sapiens (Human)	RagC,GTPase-interacting protein 2,TIB929
5	Y, N	Regulatory-associated protein of mTOR	polymer	1335	149200.0	2	UniProt (Q8N122) Pfam (PF14538) Pfam (PF02985) Pfam (PF00400) In PDB	Homo sapiens (Human)	Raptor,p150 target of rapamycin (TOR)-scaffold protein
6	T, O	Proline-rich AKT1 substrate 1	polymer	256	27412.3	2	UniProt (Q96B36) Pfam (PF15798) In PDB	Homo sapiens (Human)	40 kDa proline-rich AKT substrate
7	C, I	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
8	D, J	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)

Sequence modifications

A, B: 1 - 59 (PDB: 6SB0)
A, B: 60 - 355 (UniProt: P42345)
A, B: 356 - 380 (PDB: 6SB0)
A, B: 381 - 2549 (UniProt: P42345)
C, I: 1 - 313 (UniProt: Q7L523)

PDB	External Database	Details
Leu 66	Gln 66	engineered mutation

D, J: 1 - 399 (UniProt: Q9HB90)

PDB	External Database	Details
Asn 90	Thr 90	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	1160838.9
Non-Polymers*	Number of molecules	4
	Total formula weight	1932.8
All*	Total formula weight	1162771.7

*Water molecules are not included.