6S7O
Cryo-EM structure of human oligosaccharyltransferase complex OST-A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A | polymer | 705 | 80607.3 | 1 | UniProt (P46977) Pfam (PF02516) Pfam (PF22627) | Homo sapiens (Human) | STT3-A,B5,Integral membrane protein 1,Transmembrane protein TMC |
| 2 | B (B) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 | polymer | 37 | 4196.0 | 1 | UniProt (P0C6T2) Pfam (PF10215) | Homo sapiens (Human) | |
| 3 | C (C) | Transmembrane protein 258 | polymer | 79 | 9083.8 | 1 | UniProt (P61165) Pfam (PF05251) | Homo sapiens (Human) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258,Oligosaccharyl transferase subunit TMEM258 |
| 4 | D (D) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 | polymer | 113 | 12503.6 | 1 | UniProt (P61803) Pfam (PF02109) | Homo sapiens (Human) | Oligosaccharyl transferase subunit DAD1,Defender against cell death 1,DAD-1 |
| 5 | E (E) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 | polymer | 607 | 68656.2 | 1 | UniProt (P04843) Pfam (PF04597) | Homo sapiens (Human) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit,Ribophorin I,RPN-I,Ribophorin-1 |
| 6 | F (F) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 | polymer | 631 | 69347.5 | 1 | UniProt (P04844) Pfam (PF05817) | Homo sapiens (Human) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit,RIBIIR,Ribophorin II,RPN-II,Ribophorin-2 |
| 7 | G (G) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit | polymer | 456 | 50754.4 | 1 | UniProt (A0A024RAD5) Pfam (PF03345) UniProt (by SIFTS) (P39656) | Homo sapiens (Human) | Oligosaccharyl transferase 48 kDa subunit |
| 8 | H (H) | Oligosaccharyltransferase complex subunit OSTC | polymer | 149 | 16844.2 | 1 | UniProt (Q9NRP0) Pfam (PF04756) | Homo sapiens (Human) | Hydrophobic protein HSF-28 |
| 9 | I (I) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 10 | J (J) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1397.2 | 1 | In PDB GlyTouCan (G81980VO) | |||
| 11 | K (K) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1397.2 | 1 | In PDB GlyTouCan (G80966KZ) | |||
| 12 | AA, BA, DA, L, M... (F, G, A, E) | (2~{S},3~{R},4~{R},5~{S},6~{S})-2-(hydroxymethyl)-6-[(1~{S},2~{R},3~{R},4~{R},5'~{S},6~{S},7~{R},8~{S},9~{R},12~{R},13~{R},15~{S},16~{S},18~{R})-5',7,9,13-tetramethyl-3,15-bis(oxidanyl)spiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icosane-6,2'-oxane]-16-yl]oxy-oxane-3,4,5-triol | non-polymer | 610.8 | 9 | Chemie (KZB) | |||
| 13 | CA, O, P, S, T... (F, A, C, D, E) | (4R,7R)-4-hydroxy-N,N,N-trimethyl-4,9-dioxo-7-[(undecanoyloxy)methyl]-3,5,8-trioxa-4lambda~5~-phosphadocosan-1-aminium | non-polymer | 636.9 | 7 | Chemie (EGY) | |||
| 14 | Q, Y (A, E) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 15 | R (A) | [(3~{R},6~{Z},10~{Z},14~{Z},18~{Z})-3,7,11,15,19,23-hexamethyltetracosa-6,10,14,18,22-pentaenyl] dihydrogen phosphate | non-polymer | 508.7 | 1 | Chemie (KZE) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 311993.1 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 3381.0 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 10512.3 | |
| All* | Total formula weight | 325886.4 |
