Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6S7O

Cryo-EM structure of human oligosaccharyltransferase complex OST-A

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004576	molecular_function	oligosaccharyl transferase activity
A	0004579	molecular_function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity
A	0005515	molecular_function	protein binding
A	0005783	cellular_component	endoplasmic reticulum
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0006486	biological_process	protein glycosylation
A	0006487	biological_process	protein N-linked glycosylation
A	0008250	cellular_component	oligosaccharyltransferase complex
A	0016020	cellular_component	membrane
A	0016757	molecular_function	glycosyltransferase activity
A	0018279	biological_process	protein N-linked glycosylation via asparagine
A	0035000	cellular_component	oligosaccharyltransferase III complex
A	0043686	biological_process	co-translational protein modification
A	0043687	biological_process	post-translational protein modification
A	0046872	molecular_function	metal ion binding
B	0005515	molecular_function	protein binding
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0006486	biological_process	protein glycosylation
B	0006487	biological_process	protein N-linked glycosylation
B	0008250	cellular_component	oligosaccharyltransferase complex
B	0016020	cellular_component	membrane
B	0018279	biological_process	protein N-linked glycosylation via asparagine
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005783	cellular_component	endoplasmic reticulum
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0006486	biological_process	protein glycosylation
C	0006487	biological_process	protein N-linked glycosylation
C	0006954	biological_process	inflammatory response
C	0008250	cellular_component	oligosaccharyltransferase complex
C	0016020	cellular_component	membrane
C	0032991	cellular_component	protein-containing complex
C	0034976	biological_process	response to endoplasmic reticulum stress
C	0034998	cellular_component	oligosaccharyltransferase I complex
C	0043227	cellular_component	membrane-bounded organelle
C	0062062	molecular_function	oligosaccharyltransferase complex binding
C	1904019	biological_process	epithelial cell apoptotic process
D	0001824	biological_process	blastocyst development
D	0005783	cellular_component	endoplasmic reticulum
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0006486	biological_process	protein glycosylation
D	0006487	biological_process	protein N-linked glycosylation
D	0006915	biological_process	apoptotic process
D	0008047	molecular_function	enzyme activator activity
D	0008250	cellular_component	oligosaccharyltransferase complex
D	0016020	cellular_component	membrane
D	0018279	biological_process	protein N-linked glycosylation via asparagine
D	0031647	biological_process	regulation of protein stability
D	0043066	biological_process	negative regulation of apoptotic process
E	0006486	biological_process	protein glycosylation
E	0016020	cellular_component	membrane
F	0006487	biological_process	protein N-linked glycosylation
F	0008250	cellular_component	oligosaccharyltransferase complex
F	0016020	cellular_component	membrane
G	0005789	cellular_component	endoplasmic reticulum membrane
G	0018279	biological_process	protein N-linked glycosylation via asparagine
H	0004579	molecular_function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity
H	0005515	molecular_function	protein binding
H	0005783	cellular_component	endoplasmic reticulum
H	0005789	cellular_component	endoplasmic reticulum membrane
H	0006486	biological_process	protein glycosylation
H	0006487	biological_process	protein N-linked glycosylation
H	0008250	cellular_component	oligosaccharyltransferase complex
H	0016020	cellular_component	membrane
H	0018279	biological_process	protein N-linked glycosylation via asparagine

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	384
Details	TOPO_DOM: Lumenal => ECO:0000255

Chain	Residue	Details
G	SER43-PRO427
H	ASP105-ARG117
A	GLY190-SER191
A	THR229-SER233
A	SER288-ARG300
A	SER380-ALA385
A	TYR429-LYS453

site_id	SWS_FT_FI2
Number of Residues	19
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
G	TYR428-LEU447
H	ILE84-LEU104
H	PHE118-PHE138

site_id	SWS_FT_FI3
Number of Residues	8
Details	TOPO_DOM: Cytoplasmic => ECO:0000255

Chain	Residue	Details
G	HIS448-ASP456
H	MET139-GLY149
A	SER207-GLY211
A	GLN260-HIS267
A	LYS322-THR356
A	MET403-LEU406
A	HIS474-THR705

site_id	SWS_FT_FI4
Number of Residues	1
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:19159218

Chain	Residue	Details
F	ASN106
A	MET407-THR428
A	ASN454-PHE473
A	ALA141-ILE158
A	GLY170-THR189
A	ILE192-VAL206
A	TYR212-LEU228
A	HIS234-PHE259
A	MET268-ARG287
A	TRP357-PHE379
A	ARG386-VAL402

site_id	SWS_FT_FI5
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Chain	Residue	Details
F	LYS154
A	ASP167
A	GLU169
A	ARG405
A	TYR530

site_id	SWS_FT_FI6
Number of Residues	3
Details	SITE: Interacts with target acceptor peptide in protein substrate => ECO:0000250\|UniProtKB:B9KDD4

Chain	Residue	Details
A	ASP49
A	GLU351
A	LYS595

site_id	SWS_FT_FI7
Number of Residues	1
Details	SITE: Important for catalytic activity => ECO:0000250\|UniProtKB:B9KDD4

Chain	Residue	Details
A	ARG160

site_id	SWS_FT_FI8
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:19159218

Chain	Residue	Details
A	ASN537
A	ASN544

site_id	SWS_FT_FI9
Number of Residues	1
Details	CARBOHYD: N-linked (GlcNAc...) (high mannose) asparagine => ECO:0000269\|PubMed:19159218

Chain	Residue	Details
A	ASN548

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)