6S6Y

X-ray crystal structure of the formyltransferase/hydrolase complex (FhcABCD) from Methylorubrum extorquens in complex with methylofuran

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M	Formylmethanofuran dehydrogenase subunit A	polymer	548	58563.0	4	UniProt (A9W3R9) In PDB	Methylobacterium extorquens (strain PA1)
2	B, F, J, N	Tungsten-containing formylmethanofuran dehydrogenase, subunit B	polymer	361	37032.5	4	UniProt (A9W3S0) In PDB	Methylobacterium extorquens (strain PA1)
3	C, G, K, O	Formylmethanofuran dehydrogenase subunit C	polymer	276	27896.6	4	UniProt (A9W3R7) In PDB	Methylobacterium extorquens (strain PA1)
4	D, H, L, P	Formylmethanofuran--tetrahydromethanopterin formyltransferase	polymer	310	32280.0	4	UniProt (A9W3R8) In PDB	Methylobacterium extorquens (strain PA1)	H4MPT formyltransferase
5	J, A, I	FORMIC ACID	non-polymer		46.0	3	Chemie (FMT)
6	A	N-[4,5,7-TRICARBOXYHEPTANOYL]-L-GAMMA-GLUTAMYL-N-{2-[4-({5-[(FORMYLAMINO)METHYL]-3-FURYL}METHOXY)PHENYL]ETHYL}-D-GLUTAMINE	non-polymer		776.7	1	Chemie (MFN)
7	H, A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
8	E, M, A, I	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
9	B, L, A	CALCIUM ION	non-polymer		40.1	6	Chemie (CA)
10	J, D, L, H, P...	POTASSIUM ION	non-polymer		39.1	9	Chemie (K)
11	A, J, C, I	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
12	B, K, C, D, L...	D-GLUTAMIC ACID	non-polymer		147.1	10	Chemie (DGL)
13	B	AMINO GROUP	non-polymer		16.0	1	Chemie (NH2)
14	J, K, C, L, O...	GLUTAMIC ACID	non-polymer		147.1	8	Chemie (GLU)
15	D, P	BETA-L-ASPARTIC ACID	non-polymer		133.1	2	Chemie (IAS)
16	I	(2~{S})-3-[4-[[5-(aminomethyl)furan-3-yl]methoxy]phenyl]-2-(methylamino)propanoic acid	non-polymer		304.3	1	Chemie (L6K)
17	I	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
18	J	SODIUM ION	non-polymer		23.0	1	Chemie (NA)

Sequence modifications

C, G, K, O: 2 - 265 (UniProt: A9W3R7)

PDB	External Database	Details
His 266	-	expression tag
Gly 267	-	expression tag
Ser 268	-	expression tag
Ala 269	-	expression tag
Trp 270	-	expression tag
Ser 271	-	expression tag
His 272	-	expression tag
Pro 273	-	expression tag
Gln 274	-	expression tag
Phe 275	-	expression tag
Glu 276	-	expression tag
Lys 277	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	623088.6
Non-Polymers*	Number of molecules	58
	Total formula weight	5711.8
All*	Total formula weight	628800.4

*Water molecules are not included.